Wyniki wyszukiwania

1

Different gene expression profiles of AD293 and HEK293 cell lines that show contrasting susceptibility to apoptosis induced by overexpression of Bim L

100%

Zhang J., Chen J., Liu L., Ji C., Gu S., Ying K., Mao B.

Acta Biochimica Polonica

|

2006

|

tom 53

|

nr 3

2

Participation of leukotriene C4 in the regulation of suicidal erythrocyte death

84%

Foller M., Mahmud H., Gu S., Wang K., Floride E., Kucherenko Y., Luik S., Laufer S., Lang F.

Journal of Physiology and Pharmacology

|

2009

|

tom 60

|

nr 3

135-143

Eryptosis, the suicidal death of erythrocytes, is characterized by cell shrinkage and cell membrane scrambling with phosphatidylserine exposure at the erythrocyte surface. Eryptosis is triggered by increase in cytosolic Ca2+ concentration upon energy depletion. The present study explored the involvement of leukotrienes. Western blotting was employed to detect the cysteinyl-leukotriene receptor cysLT1, competitive immune assay to determine leukotriene release from erythrocytes, Fluo3 fluorescence to estimate cytosolic Ca2+ concentration, forward scatter to analyse cell volume and annexin V-binding to disclose phosphatidylserine exposure. As a result, erythrocytes expressed the leukotriene receptor CysLT1. Glucose depletion (24 hours) significantly increased the formation of the cysteinyl-leukotrienes C4/D4/E4. Leukotriene C4 (10 nM) increased Ca2+ entry, decreased forward scatter, activated caspases 3 and 8, and stimulated annexin V-binding. Glucose depletion similarly increased annexin V-binding, an effect significantly blunted in the presence of the leukotriene receptor antagonist cinalukast (1 µM) or the 5-lipoxygenase inhibitor BW B70C (1 µM). In conclusion, upon energy depletion erythrocytes form leukotrienes, which in turn activate cation channels, leading to Ca2+ entry, cell shrinkage and cell membrane scrambling. Cysteinyl-leukotrienes thus participate in the signaling of eryptosis during energy depletion.

3

Molecular cloning and characterization of a novel human gene containing 4 ankyrin repeat domains

84%

Li J., Ji C., Zheng H., Fei X., Zheng M., Dai J., Gu S., Xie Y., Mao Y.

Cellular and Molecular Biology Letters

|

2005

|

tom 10

|

nr 1

4

Isolation and expression pattern of RGS21 gene, a novel RGS member

84%

Li X., Chen L., Ji C., Liu B., Gu J., Xu J., Zou X., Gu S., Mao Y.

Acta Biochimica Polonica

|

2005

|

tom 52

|

nr 4

Regulators of G-protein signaling (RGS) proteins are known for the RGS domain that is composed of a conserved stretch of 120 amino acids, which binds directly to activated G-protein α subunits and acts as a GTPase-activating protein (GAP), leading to their deactivation and termination of downstream signals. In this study, a novel human RGS cDNA (RGS21), 1795 bp long and encoding a 152-amino acid polypeptide, was isolated by large-scale sequencing analysis of a human fetal brain cDNA library. Unlike other RGS family members, RGS21 gene has no additional domain/motif and may represent the smallest known member of RGS family. It may belong to the B/R4 subfamily, which suggests that it may serve exclusively as a negative regulator of αi/o family members and/or αq/11. PCR analysis showed that RGS21 mRNA was expressed ubiquitously in the 16 tissues examined, implying general physiological roles.

5

Overexpression of BimSs3, the novel isoform of Bim, can trigger cell apoptosis by inducing cytochrome c release from mitochondria

84%

Liu L., Chen J., Zhang J., Ji C., Zhang X., Gu S., Xie Y., Mao Y.

Acta Biochimica Polonica

|

2007

|

tom 54

|

nr 3

Bim is defined as the pro-apoptotic BH3-only protein of the Bcl-2 family, which is a critical sensor and mediator in the mitochondrial-dependent apoptosis. In a previous work, we have cloned a novel transcript of Bim (GenBank accession number: AY305716) from the fetal brain cDNA, which is widely expressed in some carcinoma tissues and normal human tissues. According to the sequence analysis and the newly-defined nomenclature system of Bim isoforms (Adachi et al., 2005, Cell Death Differ 2: 192), we term it BimSs3 according to its characteristic structure. The subcellular location analysis indicated that the fused protein GFP-BimSs3 is distributed in the whole cell, mainly to the nucleus. Overexpression of BimSs3 in HEK293 cells causes apoptosis (28.16 ± 1.55%) compared to the negative control (5.44 ± 2.63%). It also causes cytochrome c release from the mitochondrial fraction to the cytosolic fraction during apoptosis. Western blotting assay indicates the molecular mass of GFP-BimSs3 is approximately 31.0 kDa (GFP: 27 kDa). Hence the open reading frame of BimSs3 may initiate at the second ATG and encodes a 36 amino-acid peptide with BH3 domain.

Ograniczanie wyników

Different gene expression profiles of AD293 and HEK293 cell lines that show contrasting susceptibility to apoptosis induced by overexpression of Bim L

Participation of leukotriene C4 in the regulation of suicidal erythrocyte death

Molecular cloning and characterization of a novel human gene containing 4 ankyrin repeat domains

Isolation and expression pattern of RGS21 gene, a novel RGS member

Overexpression of BimSs3, the novel isoform of Bim, can trigger cell apoptosis by inducing cytochrome c release from mitochondria