Preferencje help
Polish version English version Język
Widoczny [Schowaj] Abstrakt
Liczba wyników

Znaleziono wyników: 13

Liczba wyników na stronie
Pierwsza strona wyników Pięć stron wyników wstecz Poprzednia strona wyników Strona / 1 Następna strona wyników Pięć stron wyników wprzód Ostatnia strona wyników

Wyniki wyszukiwania

help Sortuj według:

help Ogranicz wyniki do:
Pierwsza strona wyników Pięć stron wyników wstecz Poprzednia strona wyników Strona / 1 Następna strona wyników Pięć stron wyników wprzód Ostatnia strona wyników
1
Content available remote

Effects of pioglitazone and high-fat diet on ceramide metabolism in rat skeletal muscles

100%
Zendzian-Piotrowska M., Baranowski M., Zabielski P., Gorski J.
Journal of Physiology and Pharmacology. Supplement
|
2006
|
tom 57
|
nr 10
101-114
Ceramide is involved in the pathogenesis of insulin resistance in skeletal muscles of humans and rodents. However, there are conflicting reports in the literature on the effect of thiazolidinediones (a new class of insulin sensitizing drugs) on skeletal muscle ceramide content. Therefore, the aim of our study was to examine the effect of pioglitazone on the level of ceramide and its metabolites and on the activity of the key enzymes of ceramide metabolism in different skeletal muscle types of the rat. The experiments were carried out on rats fed either a standard chow or a high-fat diet for 21 days. Each group was divided into two subgroups: control and treated with pioglitazone for 14 days. High-fat diet increased the content of ceramide in the soleus and in the red section of the gastrocnemius, but not in the white section of the latter. The activity of neutral Mg2+-dependent sphingomyelinase and acid sphingomyelinase was simultaneously reduced in all examined muscles. Administration of pioglitazone decreased ceramide level in the soleus and in the red section of the gastrocnemius in rats fed either diet. This effect could not be attributed to decreased rate of ceramide formation from sphingomyelin or to its augmented deacylation to sphingosine. Pioglitazone treatment reduced the concentration of plasma free fatty acids in rats fed on either diet. Therefore, we conclude that the drug decreased the muscle content of ceramide by reducing its de novo synthesis. The results of our study indicate that reduction in ceramide level may be one of the mechanisms by which pioglitazone improves skeletal muscle insulin sensitivity.
2
Content available remote

Differential effects of in vivo PPAR alpha and gamma activation on fatty acid transport proteins expression and lipid content in rat liver

100%
Wierzbicki M., Chabowski A., Zendzian-Piotrowska M., Gorski J.
Journal of Physiology and Pharmacology
|
2009
|
tom 60
|
nr 1
99-106
Peroxisome proliferator-activated receptors (PPAR`s) serve as lipid sensors and when activated modify gene expression of proteins highly involved in the regulation of fatty acid metabolism. Recently, the accumulation of lipids in liver was shown to be depended on the excessive protein-mediated transmembrane transport of long chain fatty acids (LCFAs). The aim of the present study was to determine the in vivo effects of PPAR and activation at two levels: 1) on the expression of fatty acid transporters, 2) on the content and fatty acids saturation status of lipids in rats liver. PPAR agonist (WY 14,643) treatment upregulated the liver expression of FAT/CD36 (+20%, p<0.05) and did not significantly affect the content of FABPpm and FATP-1. Accordingly there was a significant increase in the content of phospholipid (+12%, p<0.05), diacylglycerol (+65%, p<0.05) and triacylglycerol (+46%, p<0.05) fractions followed PPAR activation. In contrast, pioglitazone (PPAR agonist) had no effect on the content of fatty acid transporters (FAT/CD36, FABPpm and FATP-1) as well as the content of liver lipid fractions with the exception for triacylglycerols, which have been reduced significantly (-89%, p<0.05). These findings suggest that in vivo PPAR and PPAR activation exert different effects on both the expression of fatty acid transporters and lipid content in rat’s liver.
3
Artykuł dostępny w postaci pełnego tekstu - kliknij by otworzyć plik
Content available

The influence of heavy physical effort on proteolytic adaptations in skeletal and heart muscle and aorta in rats

100%
Gilbert-Matusiak A., Wyczalkowska-Tomasik A., Zendzian-Piotrowska M., Czrkowska-Paczek B.
Annals of Agricultural and Environmental Medicine
|
2018
|
tom 25
|
nr 4
4
Content available remote

One session of exercise regulates cathepsin B activity in the livers of trained and untrained rats

100%
Wyczalkowska-Tomasik A., Czarkowska-Paczek B., Piekarczyk-Persa J., Zendzian-Piotrowska M.
Journal of Physiology and Pharmacology
|
2017
|
tom 68
|
nr 5
5
Content available remote

Effects of hyperthyroidism on lipid content and composition in oxidative and glycolytic muscles in rats

100%
Miklosz A., Chabowski A., Zendzian-Piotrowska M., Gorski J.
Journal of Physiology and Pharmacology
|
2012
|
tom 63
|
nr 4
6
Artykuł dostępny w postaci pełnego tekstu - kliknij by otworzyć plik
Content available

Effect of triiodothyronine on the content of phospholipids in the rat liver nuclei

100%
Bucki R., Gorska M., Zendzian-Piotrowska M., Gorski J.
Journal of Physiology and Pharmacology
|
2000
|
tom 51
|
nr 3
7
Content available remote

Effect of endurance training on the sphingomyelin-signalling pathway activity in the skeletal muscles of the rat

100%
Dobrzyn A., Zendzian-Piotrowska M., Gorski J.
Journal of Physiology and Pharmacology
|
2004
|
tom 55
|
nr 2
The sphingomyelin signalling pathway has been shown to function in different skeletal muscle types. The aim of the present study was to examine the effect of endurance training on the functioning of the pathway in the muscles. The experiments were carried out on two groups of male Wistar rats: sedentary and trained for six weeks. 24h after cessation of the training rats were anaesthetized and samples of the soleus, red and white section of the gastrocnemius were taken. The content and composition of sphingomyelin-fatty acids and ceramide - fatty acids was determined by means of gas-liquid chromatography. The content of sphingosine and sphinganine was determined by means of high-pressure liquid chromatography. The activity of neutral Mg++-dependent sphingomyelinase was determined spectophotometrically using trinitrophenylaminolauroyl-sphingomyelin as the substrate. It has been found that training reduces the total content of sphingomyelin- and ceramide-fatty acids, increases the content of sphinganine and does not affect the content of sphingosine in individual muscle types. The activity of the enzyme in the muscles is also elevated. It is concluded that training affects functioning of the sphingomyelin -signalling pathway in skeletal muscles. The reduction in the content of ceramide may contribute to elevation in glucose uptake in skeletal muscles observed after training.
8
Artykuł dostępny w postaci pełnego tekstu - kliknij by otworzyć plik
Content available

Effect of triiodothyronine on phospholipid metabolism in skeletal muscles of the rat

100%
Zendzian-Piotrowska M., Gorska M., Dworakowski W., Gorski J.
Journal of Physiology and Pharmacology
|
2000
|
tom 51
|
nr 1
9
Content available remote

One session of exercise or endurance training does not influence serum levels of irisin in rats

88%
Czarkowska-Paczek B., Zendzian-Piotrowska M., Gala K., Sobol M., Paczek L.
Journal of Physiology and Pharmacology
|
2014
|
tom 65
|
nr 3
10
Content available remote

The effect of acute and prolonged endurance exercise on transforming growth factor-ß1 generation in rat skeletal and heart muscle

88%
Czarkowska-Paczek B., Zendzian-Piotrowska M., Bartlomiejczyk I., Przybylski J., Gorski J.
Journal of Physiology and Pharmacology
|
2009
|
tom 60
|
nr 4
157-162
The serum level of the transforming growth factor-beta1 (TGF-ß1) is elevated after acute bouts of exercise and prolonged training, as well as after myocardial infarction. However, the source of this increase remains unclear. Contracting skeletal muscles are known to be the source of many cytokines. To determine whether skeletal or heart muscles produce TGF-ß1 during exercise, we investigated the effect of a single bout of acute exercise on TGF-ß1 generation in skeletal and heart muscles in untrained rats (UT, n=30) and in rats subjected to prolonged (6-week) endurance training (T, n=29). The UT and T (a day after final training) groups were subjected to an acute bout of exercise with the same work load. Rats from both groups were sacrificed and skeletal and heart muscle samples were collected before (pre), immediately after (0 h), or 3 hours (3 h) after acute exercise. TGF-ß1 mRNA was quantified by RT-PCR in these samples, and basal TGF-ß1 protein levels were determined in skeletal muscle in the UTpre and Tpre subgroups by ELISA. Acute exercise caused a non-significant increase in TGF-ß1 mRNA in skeletal muscle in UT0h rats, in compare to UTpre rats. There was a significant decrease of TGF-ß1 mRNA in the T0h group (p=0.0013) in compare to Tpre rats. Prolonged training caused a significant increase in TGF-ß1 mRNA (p=0.02); however, the TGF-ß1 protein level decreased (p=0.02). In heart muscle, there was a significant decrease of TGF-ß1 mRNA in UT0h (p=0.01) and UT3h (p=0.04) compared to UTpre rats. TGF-ß1 mRNA levels were unchanged in T0h and T3h compared to Tpre; basal TGF-ß1 mRNA expression after training was also unchanged (UTpre vs. Tpre). We conclude that physical exercise is a potent stimulus for inducing TGF-ß1 gene expression in skeletal muscle, but does not increase the protein level. Thus, skeletal and heart muscle do not contribute to increased serum levels of TGF-ß1 after physical exercise.
11

Ceramide and sphingomyelin levels are increased in brains of rats exposed to streptozotocin

76%
Prokopiuk S., Fiedorowicz A., Bienias K., Sadowska A., Niemirowicz K., Zendzian-Piotrowska M., Car H.
Acta Neurobiologiae Experimentalis
|
2013
|
tom 73
|
nr 1
Insulin insufficiency and increased glucose levels are the major features of diabetes type 1 leading to cognitive dysfunctions and neurodegeneration. A reason why different brain structures are characterized by diverse response to increased glucose level is not known. Our previous study showed increased ceramide levels in the brains of rats with diabetes induced by streptozotocin (STZ) injection, which was abolished by myriocin, the inhibitor of serine palmitylotransferase. Ceramides may be important mediators of neuropathological changes and its elevation was found in many brain disorders. The main goal of our present study was to verify if hippocampus, prefrontal cortex and cerebellum response differently to hyperglycemia/hypoinsulinemia in terms of changes in sphingolipids concentrations. We attempted to identify potential source of ceramides by measuring the sphingomyelinase concentrations and by blocking the ceramide de novo synthesis pathway. We found that in cerebellum and hippocampus of hyperglycemic animals sphingolipids concentrations underwent subtle modifications while prefrontal cortex exhibited massive changes in ceramides and SMs content. Total ceramide levels was significantly elevated in prefrontal cortex of diabetic rats, which was reduced by myriocin, while rats exposed to STZ showed only small increase of total SM in this brain structure. The increased content of ceramides containing SAFAs (saturated fatty acids) in prefrontal cortex was diminished by myriocin. SAFA-contained SMs did not present changes. Elevation of MUFA- (monounsaturated fatty acids), and PUFA-ceramides (polyunsaturated fatty acids) in prefrontal cortex of STZ-treated rats was reduced by myriocin, similarly as MUFA-SMs augmentation. PUFA-ceramides and PUFA-SMs experienced only slight modifications. Both – ceramides and omega-6 – SMs increased dramatically and were downregulated by myriocin. We conclude that the prefrontal cortex may be particularly sensitive to hyperglycemic conditions and hypoinsulinemia. Moreover, the novo synthesis seems to be an important pathway of ceramide generation since usage of myriocin strongly reduced ceramide levels enhanced by STZ injection. Augmentation in ceramide content was correlated with enhancement of SMs production. These unexpected results may be explained by the incorporation of redundant ceramides into SMs, a mechanism by which the toxic level of ceramides is reduced in the brain. Supported by grant 123-27575 P from the State Committee for Scientific Research, Warsaw, Poland
12
Artykuł dostępny w postaci pełnego tekstu - kliknij by otworzyć plik
Content available

Inequalities in breast cancer incidence and stage distribution between urban and rural female population in Swietokrzyskie Province, Poland

64%
Paszko A., Krzyzak M. J., Charkiewicz A. E., Ziembicka D., Zendzian-Piotrowska M., Szpak A. S., Florek-Luszczki M., Maslach D.
Annals of Agricultural and Environmental Medicine
|
2019
|
tom 26
|
nr 1
13

Brain spingolipids in hyperglycemia

52%
Car H., Fiedorowicz A., Prokopiuk S., Bienias K., Zendzian-Piotrowska M., Chabowski A., Kosacka I., Tidhar R., Futerman A. H., Glombik K., Basta-Kaim A.
Acta Neurobiologiae Experimentalis
|
2015
|
tom 75
|
nr Supl.
Ceramide (Cer) and sphingomyelin (SM) are members of sphingolipid (SL) family. Their concentrationsin the brain undergo substantial changes in many pathologies. They are also important players in diabetes-linked brain dysfunctions, in which increased content of ceramides can be toxic to neurons. The aim of the study was to evaluate selected parameters of sphingolipids and insulin pathways in prefrontal cortex (PC) and hippocampus (H) of rats with experimentally induced hyperglycemia. STZ-rat model of type 1 diabetes and high fat diet model of insulin resistance were used. Analyses of studied parameters were performed by GLC, IHC and Elisa. We found the augmented levels of ceramides in H and PC and only minor in striatum and cerebellum of rats with STZ-induced diabetes. Similar expressions of Cer were confirmed by IHC. Myriocin, an inhibitor of an enzyme of ceramide de novo synthesis pathway, reduced ceramide generation in hyperglycemic brains, particularly in PC, which was reflected in altered Cer synthase activities. In addition, we reported the fluctuations in sphingomyelin levels in investigated structures. The level of insulin did not change in H and PC of STZ-treated rats. An expression of insulin receptor and its phosphorylated form decreased in both structures, but was restored after myriocin administration. Similarly, Akt and phosphorylated Akt changed in these structures suggesting important role of de novo Cer synthesis in intracellular pathways of insulin. In the rat model of high fat diet, which leads to insulin resistance, the sphingolipid pattern (Cer and SM) was altered in H and PC as well. Metformin, the drug of choice in diabetes type 2, influenced the content of the above SLs in these structures, suggesting the additional central activity of antidiabetic treatment. We conclude that ceramide and SM may be important mediators of diabetes- accompanied brain dysfunction.
Pierwsza strona wyników Pięć stron wyników wstecz Poprzednia strona wyników Strona / 1 Następna strona wyników Pięć stron wyników wprzód Ostatnia strona wyników
JavaScript jest wyłączony w Twojej przeglądarce internetowej. Włącz go, a następnie odśwież stronę, aby móc w pełni z niej korzystać.