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1
Artykuł dostępny w postaci pełnego tekstu - kliknij by otworzyć plik
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Chemical composition, antimicrobial and antioxidant activities of Dittrichia graveolens (L.) Greuter essential oil

100%
Mahboubi M.
Herba Polonica
|
2011
|
tom 57
|
nr 3
The aim of the study was to survey the chemical composition, antimicrobial and antioxidant activities of Dittrichia graveolens essential oil in in vitro conditions. GC-FID and GC-MS analyses were performed to evaluate the chemical composition of essential oil. The antimicrobial activity against different kinds of microorganisms was determined by microbroth dilution assay. The antioxidant activity was evaluated by DPPH free radical scavenging system. Studies of chemical composition of essential oil revealed the presence of borneol (38.2%) and bornyl acetate (14.9%) as major constituents. Essential oil showed strong antimicrobial activity against Enterococcus faecalis isolates and Bacillus cereus. Staphylococcus aureus and Escherichia coli are less sensitive to the oil. The strong antimicrobial activity of oil against clinical isolates of bacteria was demonstrated. In addition, D. graveolens oil notably reduced the concentration of DPPH free radical with higher efficacy than that of trolox at 70 min.
2
Artykuł dostępny w postaci pełnego tekstu - kliknij by otworzyć plik
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Antimicrobial and antioxidant activity of Epimedium pinnatum

71%
Mahboubi M., Kazempour N., Hosseini H., Mahboubi M.
Herba Polonica
|
2013
|
tom 59
|
nr 2
Epimedium pinnatum (Berberidaceae family) is used as an aphrodisiac in traditional Chinese medicine (TCM). The aim of this study was to evaluate the antimicrobial and antioxidant activity of E. pinnatum extracts (ethanol, methanol and aqueous extracts). Total phenolic (TPC) and flavonoid contents (TFC) of each extract were assessed by spectrophotometric methods. It was exhibited that methanol extract had better antimicrobial activity than those of ethanolic extract or aqueous extract. The TPC and TFC of E. pinnatum extracts was higher in methanol extract (149 and 36.6 mg/g) than that of ethanolic extract (137.2 and 19.5 mg/g) and aqueous extract (86.2 and 8.4 mg/g). The methanol extract had lower IC50 value (200 µg/ml) than ethanolic (250 µg/ml) and aqueous extract (400 µg/ml). There was a positive correlation between TPC, TFC in E. pinnatum extract and their antioxidant and antimicrobial activity.
3
Artykuł dostępny w postaci pełnego tekstu - kliknij by otworzyć plik
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Antimicrobial activity of Capparis spinosa as its usages in traditional medicine

63%
Mahboubi M., Mahboubi A.
Herba Polonica
|
2014
|
tom 60
|
nr 1
Capparis spinosa roots and fruits are traditionally used for the treatment of diseases such as diarrhea and hemorrhoids. In the 10th century, farmers also used aqueous extract from its roots for disinfecting their plants’ seeds. Due to widespread use of this plant in traditional medicine, we evaluated different extracts (aqueous, methanol, ethanol and ethyl acetate) of fruits and roots of this plant against bacteria and fungi causing infections in plants or humans. The total phenolic and flavonoid content of extracts from fruits and roots and the antioxidant activity were evaluated. The antimicrobial activity was evaluated by microdilution assay in fluid medium. Among different extracts from roots and fruits, root ethyl acetate and fruit methanol extracts had higher total phenolic content, while fruit ethyl acetate extract contained higher total flavonoid content. The higher antioxidant activity was shown for roots ethanol extract (IC50=88 μg/ml). As its traditional uses, roots aqueous extract from C. spinosa exhibited inhibitory effect against bacteria and fungi with the exception of Pseudomonas aeruginosa. With the exception of fruit aqueous extract, other extracts (methanol, ethanol and ethyl acetate) from fruit and root exhibited good activity against microorganisms, especially fungi. This study reveals the traditional uses of roots and fruit extracts as antimicrobials agent.
4
Artykuł dostępny w postaci pełnego tekstu - kliknij by otworzyć plik
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The antimicrobial and antioxidant activity of Muscari neglectum flower ethanol extract

63%
Mahboubi M., Taghizadeh M.
Herba Polonica
|
2016
|
tom 62
|
nr 4
Introduction: Muscari neglectum has been used as food in some countries. Objective: The antimicrobial and antioxidant activities of M. neglectum were the subject of the study. Methods: In this study, the total phenolic, flavonoid, monomeric anthocyanin contents, antioxidant and antimicrobial activity of M. neglectum flowers ethanol extract were determined by different methods against some food poisoning microorganisms. Results: Total phenolic, flavonoids and anthocyanin contents of the extract were 18.2, 0.94 and 0.11%, respectively. The IC50 for M. neglectum was higher than that of BHT. M. neglectum extract showed no inhibition zone (IZ) against Staphylococcus aureus and had small IZ against Candida albicans and Aspergillus niger. Shigella flexneri and Escherichia coli had the higher IZ than the others. The lower MIC and MLC values were for C. albicans, followed by Sh. flexneri and E. coli. S. aureus had the higher MIC and MLC values than the others. Conclusion: Therefore, the M. neglectum flower extract can be used as a natural preservative and coloring agent in foods as replacement of synthetic ones.
5

Biological activity of essential oil from aerial parts of Artemisia aucheri Boiss. from Iran

63%
Mahboubi M., Bidgoli F. G.
Herba Polonica
|
2009
|
tom 55
|
nr 4
96-104
Artemisia aucheri Boiss. is a shrub from Asteraceae family has been widespread in Iran. In traditional medicine, A. aucheri is used for its astringent, disinfectant, antimicrobial and antiparasitic properties. The aim of this study was to evaluate the chemical composition and antimicrobial activity of essential oil from aerial parts of A. aucheri against different microorganisms including Gram positive, Gram negative bacteria, filamentous fungi and yeast by disc diffusion and micro broth dilution assays. The antioxidant activity of A. aucheri essential oil was evaluated by DPPH free radical scavenging system. Fifty five components were identified by GC and GC/MS analysis and quantified from the essential oil of A. aucheri, representing 98% of total oil. The major components were geranyl acetate (17.2%), E-citral (17.1%), linalool (12.7%), geraniol (10.7%), Z-citral (10.5%). The antimicrobial results showed that Pseudomonas aeruginosa was resistant to the oil and Staphylococcus aureus and Candida albicans showed the best sensitivity to the oil. The A. aucheri has powerful antioxidant activity than that of Trolox. Some investigations were be done for evaluating the efficacy of essential oil.
6
Artykuł dostępny w postaci pełnego tekstu - kliknij by otworzyć plik
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Chemical composition and antifungal activities of Ziziphora tenuior and Z. clinopodioides essential oils against dermatophytes

51%
Mahboubi M., Tabar R. H., Mahdizadeh E.
Herba Polonica
|
2018
|
tom 64
|
nr 2
Ziziphora species are traditionally used for treatment of different infectious and non-infectious diseases as antiseptic agents. The aim of this study was to evaluate the chemical composition of Ziziphora clinopodioides and Z. tenuir essential oils and their antifungal effects againt five strains of dermatophytes. GC and GC-MS methods were used for essentional oils analize. The anti-elastase activities were determined by porcine pancreatic elastase assays. 48 different compounds were identified in these two essential oils, which thymol, p-cymene, 1,8-cineole and γ-terpinene were their major components. The anti-dermatophyte activities of essential oils against dermatophytes showed that the essential oils (150 ppm) inhibited the mycelium growth, about 5–100%, which Z. clinopodioides essential oil had higher mycelium growth inhibition (28–100%) than that of Z. tenuir oil. The MIC and MFC values of essential oils were 0.01–1 μl/ml. 0.5 μl/ml essential oils inhibited porcine pancreatic elastase, dose-dependently. Due to the anti-dermatophyte and anti-elastase effects of Ziziphora sp., it can be considered as natural antifungal agent for more clinical and pre-clinical trials.
7
Artykuł dostępny w postaci pełnego tekstu - kliknij by otworzyć plik
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The antimicrobial activity of Prunella vulgaris extracts

51%
Mahboubi M., Mahboubi A., Kazempour N.
Herba Polonica
|
2015
|
tom 61
|
nr 1
Prunella vulgaris ( Labiatae family) or self-heal is traditionally used for different ailments such as eye pain and inflammation, headache, dizziness, sore throat and wound healing. Total phenolic and total flavonoid contents of extracts (methanol, ethanol and aqueous) were determined by a spectrophotometer. The antimicrobial activity was evaluated by micro broth dilution assay. The total phenolic content of P. vulgaris extracts were higher in aqueous extract (156.5 mg GAC/g) followed by ethanol extract and methanol extract. The TFC content of P. vulgaris methanol extract (82.8 mg QE/g) was higher than ethanol extract (22.7 mg QE/g) and aqueous extract (16.2 mg QE/g). The antimicrobial activity of methanol or ethanol extracts was higher than aqueous extract from P. vulgaris. The sensitivity of microorganisms to different extracts is related to type of pathogens. There is no positive relation between total phenolic content and its antimicrobial activity. Prunella vulgaris ethanolic extract as a source of phenolic and flavonoid contents can be used as an antimicrobial agent
8
Artykuł dostępny w postaci pełnego tekstu - kliknij by otworzyć plik
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Chemical composition, antioxidant and antimicrobial activity of Nepeta persica Boiss. essential oil

51%
Mahboubi M., Kazempour N., Ghazian F., Taghizadeh M.
Herba Polonica
|
2011
|
tom 57
|
nr 1
The essential oil from aerial parts of Nepeta persica Boiss was obtained by steam distillation and was analyzed by GC and GC/MS. Fourteen components were identified as accounting for 97.3% of total oil composition. The 4aα,7α,7aα-nepetalactone (80%) and Spiro[5.6]dodecane (14.2%) were the main components of essential oil. Antimicrobial activity of essential oil against different kinds of microorganisms was determined by micro-broth dilution assay. The minimal inhibitory concentration and minimal lethal concentration values of oil were in the ranges from 1-8 to 1-16 μl/ml, respectively. Most of all, the oil was sensitive to Candida albicans, Staphylococcus aureus and Klebsiella pneumoniae. Antioxidant activity was evaluated by 2, 2’-diphenyl-1-picrylhydrazyl (DPPH) free radical scavenging. The IC50 values of N. persica essential oil and BHT were nearly 0.03%, 2.98%, respectively and in β-carotene/linoleic acid system, the essential oil did not show effective antioxidant activity.
9
Artykuł dostępny w postaci pełnego tekstu - kliknij by otworzyć plik
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Antibacterial activity of Zataria multiflora essential oil and its main components against Pseudomonas aeruginosa

51%
Mahboubi M., Heidarytabar R., Mahdizadeh E.
Herba Polonica
|
2017
|
tom 63
|
nr 3
Introduction: In Iranian traditional medicine, Zataria multiflora Boiss (Lamiaceae family) is reputed due to its antiseptic effects. Objective: The purpose of this study was to evaluate the antibacterial and biofilm killing effects of Z. multiflora essential oil and main components against Pseudomonas aeruginosa. Methods: The main components of essential oil were identified by gas chromatography (GC) and gas chromatography–mass spectrometry (GC-MS). The antibacterial properties of Z. multiflora oil and main components were determined by assessing the MIC and MBC values, and their inhibition percent of biofilm killing effects were determined by the evaluation of optical density. The role of each main component in these activities was determined according to the chemical profiles of essential oil. Results: Thymol (38.7%), carvacrol (30.6%), and p-cymene (8.3%) were main components of twenty five components of essential oil. Carvacrol had the higher role in antibacterial activity against P. aeruginosa, followed by thymol. P-cymene enhanced the antibacterial activities of thymol and carvacrol against P. aeruginosa. Carvacrol showed the weak role in biofilm killing effect. In spite of the low antibacterial activity of p-cymene against P. aeruginosa, it can enhance the antibacterial activity of thymol or carvacrol. Conclusion: Z. multiflora essential oil can be used for the management of P. aeruginosa infections. Determining the precise role of each components needs investigating in their behavior in different media.
10
Artykuł dostępny w postaci pełnego tekstu - kliknij by otworzyć plik
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Antibacterial activity of Tribulus terrestris methanol extract against clinical isolates of Escherichia coli

51%
Batoei S., Mahboubi M., Yari R.
Herba Polonica
|
2016
|
tom 62
|
nr 2
Introduction: Tribulus terrestris L. is traditionally used for treatment of urinary tract infections. Escherichia coli, as the most prominent agent of urinary tract infections, can be sensitive to T. terrestris extract. Objectives: The aim of this study was to evaluate the antibacterial activity of T. terrestris methanol extract against clinical isolates of E. coli from urinary tract infections. Saponins were determined as main constituents of T. terrestris methanol extract. Methods: The antibacterial activities of T. terrestris methanol extract were evaluated by micro-broth dilution assay. The synergistic effects of T. terrestris methanol extract were screened with gentamicin by micro titer plate and disc diffusion assays. The isobologram curve was figured and the Fractional Inhibitory Concentration Index (FICI) was determined. Results: The saponin content of T. terrestris methanol extract was 54% (w/w). The means of MIC and MBC values for E. coli clinical isolates (n=51) were 3.5±0.27 and 7.4±0.5 mg/ml while these amounts were 3.9±1.3 and 6.4±1.8 μg/ml for gentamicin. T. terrestris methanol extract and gentamicin had synergistic effect with FICI equal to 0.1375. Conclusion: Therefore, T. terrestris can be applicable as alternative treatment in management of urinary tract infections.
11

Antimicrobial activity of essential oil from Oliveria decumbens and its synergy with vancomycin against Staphylococcus aureus

45%
Mahboubi M., Mohammadi-Yeganeh S., Bokaee S., Dehdashti H., Feizabadi M. M.
Herba Polonica
|
2007
|
tom 53
|
nr 4
Oliveria decumbens Vent (Umbelliferae) is a shrub commonly found in the South-East of Iran. Its aerial section is extensively used in herbal medicine. The Disk Diffusion Test and Microbroth Dilution Assay were used to determine the antimicrobial activity of the essential oil from Oliveria decumbens Vent against Staphylococcus aureus. To detect synergy, vancomycin was added to Mueller-Hinton agar at sub-inhibitory concentrations and the inhibitory zones were recorded in millimeters. The main components of oil were thymol (22%), carvacrol (22%) and p-cymene (19%). The O. decumbens oil exhibited strong antistaphylococcal activity (18.0±0.86). Carvacrol was considerably more effective (29.8±1.5) than thymol (17.2±1.13) and p-cymene (0.0±0.0) against Staphylococcus aureus. The oil presented strong synergism with vancomycin (24.9±0.75 vs. 19.3±0.54, p<0.001). However, further studies are required to evaluate its in vivo efficacy.
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