This paper analysed the impact of rapeseed size and storage conditions on content of phosphorus and phospholipids in seeds and oils. The study was carried out with variably-sized seed fractions of „Bios” rapeseed cultivar and the extracted oils. The seed and oil samples were tested for phosphorus content and the phospholipid profiles. Seed samples were stored for one year at ambient temperature (20±4℃) and at refrigerating temperature (7±1℃). The fine seed fraction (2.0–1.6 mm) and extracted oils from this sample had the highest total phosphorus content, phospholipid proportion and total share of their non-hydratable form, such as phosphatidic acid, and phos-phatidylethanolamine. The assumed method of storing seeds at ambient temperature and refrigerating temperature practically did not affect the phosphorus content or the phospholipid proportions inthe majority of the seed and oil samples. On the other hand, the temperature of storage had different impact of the proportion of individual phospholipids in phospholipids profile of seeds and oils