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Combined use of linked markers for genotyping the Pm1 locus in common wheat

100%

Stepien L., Chelkowski J., Wenzel G., Mohler V.

Cellular and Molecular Biology Letters

2004

tom 09

nr 4B

819-827

Genotyping of 98 wheat cultivars/lines was carried out with molecular markers that are linked to the Pm1 locus: two bi-allelic (dominant) markers: the sequence-tagged site Xsts638-7A and the amplified fragment length polymorphism XE39M58-77-7A; and the multi-allelic simple sequence repeat marker Xgwm344-7A. Employing segregation data recorded in the population Chinese Spring x Virest (Pm1e), genetic mapping revealed that Xgwm344-7A and XE39M58-77-7A were distally linked to Pm1e in the repulsion phase with respective linkage distances of 0.9 cM and 4.8 cM, while Xsts638-7A was found to co-segregate with Pm1e in the coupling phase. The genotyping results of Xsts638-7A and XE39M58-77-7A confirmed disease scoring, except for the accessions of cultivars Omega, Remus and Weihenstephan Stamm M1N. The SSR marker Xgwm344 amplified 15 different fragments ranging from 102 bp to 147 bp, with 15 entries being null-allelic at the 7A and 7B homoeoloci. It was found that wheat lines having resistance alleles at the Pm1 locus mainly show the null allele at the Xgwm344-7A locus. Due to their fast-evolving nature, the use of multi-allelic SSRs for genotype determination may be complicated. However, the combined use of multiple linked marker alleles seems to be a promising approach for genotyping a broad range of plant materials.

Plant breeding at the onset of the 3RD millenium

88%

Wenzel G., Frei U., Lubberstedt T., Mohler V., Thummler F.

Plant Breeding and Seed Science

2001

tom 45 Suppl.

Chromosomal location and molecular mapping of a tan spot resistance gene in the winter wheat cultivar Red Chief

76%

Tadesse W., Schmolke M., Hsam S. L. K., Mohler V., Wenzel G., Zeller F. J.

Journal of Applied Genetics

2010

tom 51

nr 3

The winter wheat cultivar Red Chief has been identified as the wheat cultivar most resistant to Pyrenophora tritici-repentis (Ptr). This study was undertaken to determine the inheritance, chromosomal location and molecular mapping of a tan spot resistance gene in Red Chief, χ² analysis of the F₂ segregation data of the hybrids between 21 monosomic lines of the susceptible wheat cultivar Chinese Spring and the resistant cultivar Red Chief revealed that tan spot resistance in cv. Red Chief is controlled by a single recessive gene located on chromosome 3 A. Linkage analysis using SSR markers in the Red Chief/Chinese Spring F₂ population showed that the tsr4 gene is clustered in the region around Xgwm 2a, on the short arm of chromosome 3 A. This marker has also been identified as the closest marker to the tsr3 locus on chromosome 3D in synthetic wheat lines. Validation analysis of this marker for the tsr3 and tsr4 genes using 28 resistant and 6 susceptible genotypes indicated that the 120 bp allele (the tsr3 gene) specific fragment was observed in 11 resistant genotypes, including the three synthetic lines XX41, XX45 and XX110, while the 130 bp allele was amplified only in cv. Red Chief and Dashen. Xgwm2a can be used to trace the presence of the target gene in successive backcross generations and pyramiding of the tsr3 & tsr4 genes into a commonly grown and adaptable cultivar.

Ograniczanie wyników

1 Cellular and Molecular Biology Letters

1 Journal of Applied Genetics

1 Plant Breeding and Seed Science

3 Mohler V.

3 Wenzel G.

1 Chelkowski J.

1 Frei U.

1 Hsam S. L. K.

1 Lubberstedt T.

1 Schmolke M.

1 Stepien L.

1 Tadesse W.

1 Thummler F.

1 Zeller F. J.

1 2010

1 2004

1 2001

Wyniki wyszukiwania

Combined use of linked markers for genotyping the Pm1 locus in common wheat

Plant breeding at the onset of the 3RD millenium

Chromosomal location and molecular mapping of a tan spot resistance gene in the winter wheat cultivar Red Chief