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Production, characterization and iron binding affinity of hydroxamate siderophores from rhizosphere associated fluorescent Pseudomonas

100%

Deori M., Jayamohan N. S., Kumudini B. S.

Journal of Plant Protection Research

2018

tom 58

nr 1

Fluorescent Pseudomonas (FP) is a major group of plant growth promoting rhizobacteria and a well-known synthesizer of siderophores, which imparts a selective advantage on rhizosphere competence and their biocontrol traits. The present study was aimed at examining the factors affecting the production of siderophores and their potential biocontrol traits. Sixteen FP isolates were shortlisted based on their siderophore-producing ability in chrome azural S medium. The isolates were checked for variations in siderophore production under varying incubation times, temperatures, pH, iron (Fe3+) concentrations and mutagens. In addition, the iron binding affinity of siderophores, mycelial inhibition assay and plant growth promotion traits were assessed. Results showed that the siderophore production was highly influenced by the time of incubation, changes in pH, temperature and iron concentration. Chemical characterization showed that the produced siderophores were hydroxamates. Maximum siderophore production was observed at pH 7 whereas UV and EtBr exposure invariably suppressed siderophore production drastically in all isolates. All FPs from maize rhizosphere showed excellent siderophore production which could be due to the competence in strategy-II of the plant rhizosphere and significant growth inhibition on Fusarium oxysporum. Our results suggest the inclination of siderophores to iron, in terms of various criteria affecting production and the possible role of environmental mutations that affect the natural iron harvesting mechanism.

Production and characterization of alpha-amylase from Aspergillus niger JGI 24 isolated in Bangalore

76%

Varalakshmi K. N., Kumudini B. S., Nandini B. N., Solomon J., Suhas R., Mahesh B., Kavitha A. P.

Polish Journal of Microbiology

2009

tom 58

nr 1

29-36

Five fungal isolates were screened for the production of α-amylase using both solid-state and submerged fermentations. The best amylase producer among them, Aspergillus niger JGI 24, was selected for enzyme production by solid-state fermentation (SSF) on wheat bran. Different carbon and nitrogen supplements were used to enhance enzyme production and maximum amount of enzyme was obtained when SSF was carried out with soluble starch and beef extract (1 % each) as supplements. Further attempts to enhance enzyme production by UV induced mutagenesis were carried out. Survival rate decreased with increase in duration of UV exposure. Partial purification of the enzyme using ammonium sulphate fractionation resulted in 1.49 fold increase in the enzyme activity. The enzyme showed a molecular weight of 43 kDa by SDS-PAGE. Metal ions Ca²⁺ and Co²⁺ increased the enzyme activity. The enzyme was optimally active at 30°C and pH 9.5.

Ograniczanie wyników

1 Journal of Plant Protection Research

1 Polish Journal of Microbiology

2 Kumudini B. S.

1 Deori M.

1 Jayamohan N. S.

1 Kavitha A. P.

1 Mahesh B.

1 Nandini B. N.

1 Solomon J.

1 Suhas R.

1 Varalakshmi K. N.

1 2018

1 2009

Wyniki wyszukiwania

Production, characterization and iron binding affinity of hydroxamate siderophores from rhizosphere associated fluorescent Pseudomonas

Production and characterization of alpha-amylase from Aspergillus niger JGI 24 isolated in Bangalore