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1

Characterization and mass spectrometry analysis of aminopeptidase N from Pseudomonas putida Lup

100%
Jankiewicz U., Swiontek-Brzezinska M., Gorska E. B., Kowalczyk P.
Polish Journal of Microbiology
|
2013
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tom 62
|
nr 4
An intracellular aminopeptidase N synthesized by Pseudomonas putida Lup was purified and characterized. The approx. 150-fold purified enzyme showed highest activity against A-β-naphthylamide at pH 7.5 and at temperature 40°C and was 100% thermostable for 240 min at 40°C. P. putida lup aminopeptidase N is a monomer with molecular mass approx. 99 kDa determined by SDS-PAGE and gel permeation chromatography. The enzyme has broad substrate specificity, but is the most active against protein substrates with N-terminal alanine and arginine. The activity of P. putida Lup aminopeptidase N is strongly inhibited in the presence of specific metallopeptidase inhibitors and is partly recovered in the presence of Zn²⁺ and Co²⁺ ions. Co²⁺, Mg²⁺ and Ca²⁺ ions increased the activity of the enzyme. Moreover, the enzyme was inhibited by inhibitors of cysteine enzymes. Analysis of fragments of the amino acid sequence of the purified enzyme demonstrated high similarity to PepN of Pseudomonas putida GB-1.
2

Rola oczyszczalni hydrobotanicznych w oczyszczaniu sciekow z terenow wiejskich

100%
Lalke-Porczyk E., Swiontek-Brzezinska M., Donderski W.
Woda - Środowisko - Obszary Wiejskie
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2010
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tom 10
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nr 3[31]
119-127
3

Microbiological and enzymatic activity of soil after pollution with fluorine

100%
Szostek R., Ciecko Z., Walczak M., Swiontek-Brzezinska M.
Polish Journal of Environmental Studies
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2015
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tom 24
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nr 6
4

Biotransformacja materii organicznej pzrez bakterie epifityczne

100%
Lalke-Porczyk E., Swiontek-Brzezinska M., Donderski W.
Acta Agraria et Silvestria. Series Agraria
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2006
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tom 49
The accomplished research covered the number and production of the heterotrophic bacteria colonizing the submerged surfaces of the two species of macrophytes: Common Reed (Phragmites australis (Cav.) Trin. ex Steudel) and Hornwort (Ceratophylhim demersum L.). These macrophytes predominate in the littoral zone of the Chelmżyńskie Lake. The ability of the bacteria to grow in the presence of different groups of amino acids and carbohydrates and decompose some biopoly- mers was determined. The greatest number and the highest production rate of the epiphytic bacteria were established to occur in the summer on the reed shoots. Amino acids and carbohy­drates stimulated the development of bacteria isolated from the rigid hornwort's surface consid­erably more strongly; in turn, bacteria covering the reed shoots hydrolyzed macromolecular compounds to a greater degree.
5

Sanitary quality of soil in and near municipal waste landfill sites

100%
Kalwasinska A., Swiontek-Brzezinska M., Burkowska A.
Polish Journal of Environmental Studies
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2012
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tom 21
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nr 6
The present study was aimed at determining the relationship between the operation of municipal landfills and the fluctuations of bacterial indicators of the sanitary quality of soil. Soil samples were collected at monthly intervals from May 2010 to April 2011 at ten sampling sites located on the premises and in vicinity of the reclaimed Municipal Landfill Site (MLS) and the new Municipal Solid Waste Management Plant in Toruń (WMP). Bacteriological analyses (the titre of coliforms, the titre of Clostridium perfringens, and the presence of Salmonella) were conducted according to the Microbiological and parasitological guidelines for the assessment of the sanitary quality of soil issued by the Institute of Rural Health in Lublin. The results obtained led to distinguishing three zones of impact of the facilities on soil. Good sanitary quality of soil was determined 100 m east of the edges of the landfill cells.
6

Chitinolytic activity of bacteria and fungi isolated from shrimp exoskeletons

100%
Swiontek-Brzezinska M., Lalke-Porczyk E., Donderski W.
Oceanological and Hydrobiological Studies
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2007
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tom 36
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nr 3
7

The chitinolytic bacterium Bacillus licheniformis M3 and its potential application in biological plant protection

88%
Jankiewicz U., Kozlowska M., Swiontek-Brzezinska M., Nowosielski J., Stepniewska-Jarosz S.
Electronic Journal of Polish Agricultural Universities. Series: Biotechnology
|
2016
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tom 19
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nr 2
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