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Large errors of low-cost MEMS inertial measurement unit (MIMU) lead to huge navigation errors, even wrong navigation information. An integrated navigation system for unmanned vessel is proposed. It consists of a low-cost MIMU and Doppler velocity sonar (DVS). This paper presents an integrated navigation method, to improve the performance of navigation system. The integrated navigation system is tested using simulation and semi-physical simulation experiments, whose results show that attitude, velocity and position accuracy has improved awfully, giving exactly accurate navigation results. By means of the combination of low-cost MIMU and DVS, the proposed system is able to overcome fast drift problems of the low cost IMU
As the main predator and disperser of seeds, rodents play an important role in the process of vegetation regeneration by adopting different foraging and hoarding strategies in forest ecosystems. Infrared automated detection cameras and seed-tagging methods were used to understand the effects of rodents on seeds in natural environments. We chose the dominant species Apodemus peninsulae (Korean field mouse) as the focus of this study, and seeds of the three species Pinus koraiensis, Corylus mandshurica and Quercus mongolica were released and tracked in a temperate forest in northeast China. The results showed that approximately 80% of the seeds were manipulated by A. peninsulae, 15.1% of the seeds were used as food, 20.4% of the seeds were handled after feeding, 41.3% of the seeds were handled during storage, and 23.3% of the seeds remained intact. In addition, A. peninsulae preferred Q. mongolica (85.3%) and P. koraiensis (85.6%) over C. mandshurica (59.2%). The rodents frequently hoarded seeds from every species in many small, close-range, widely dispersed, single scatter-hoarded caches around the seed station. Most caches were dispersed approximately 2–4 m from the seed station. The results indicated that A. peninsulae adopted significant discriminatory processing strategies for predation, consumption, dispersal and hoarding of the different seeds of sympatric species. Seed size, proportion of kernel mass, nutrient content, and hull thickness characteristics affected the scatter-hoarding decision processes.
The seedlings of two soybean genotypes, Al-tolerant PI 416937 (PI) and Al-sensitive Young, were cultured in the solution containing 0, 25 or 50 µM Al (AlCl₃‧6H₂O) for 24, 36 or 48 h in the hydroponics, and the calluses induced from two genotypes were cultured in medium containing 0, 10, 50 or 100 µM Al for 5, 10 or 15 days, respectively. The effects of Al on growth of seedling roots and calluses, antioxidant enzyme activities of superoxide dismutase (SOD) and peroxidase (POD) and lipid peroxidation were investigated. Under Al stress, PI was more tolerant to Al toxicity than Young at both intact plant and tissue levels and lower concentrations of Al significantly stimulated the root and callus growth of PI. Al application enhanced the activities of SOD and POD and lipid peroxidation in both roots and calluses of two genotypes. Although the differences of SOD activities between two genotypes in response to Al toxicity depended on Al concentration and durations of treatment, SOD activities in the roots of PI were higher than those in the roots of corresponding Young in the presence of Al for 36 or 48 h. Meanwhile, the POD activities in PI roots increased as the Al levels and durations of treatment increased, significantly higher than those in the corresponding Young roots. Moreover, Al-treated PI had significantly lower lipid peroxidation than Young at both root and callus levels. These results suggest that the enhanced antioxidant-related enzyme activities and reduced lipid peroxidation in PI might be one of Al-tolerant mechanisms.
For more efficient utilization of agricultural organic waste composts, this research formulates the demand of agricultural organic waste composts and the supply of compost converted from agricultural organic waste in the region, and models the demand-supply balance of agricultural organic waste compost in some regions. Objects of study include 6 urban districts, 5 county-level cities, and 21 counties in northern Henan Province, China. The crops’ latent demand for compost and the latent supply of compost converted from agricultural organic wastes (straw and livestock manure) in each region is computed for an analysis of demand-supply balance. According to the findings, there is a large deviation in both the ratio of the supply of livestock manure composts to that of straw composts and the demand-supply ratio of composts in the studied regions. To be specific, the overall demand-supply ratio in the studied regions is about 1.21, showing that demand is greater than supply. Theoretically, all agricultural organic wastes can be used for agricultural production in these regions. In addition, actual demand ratio (actual demand/ latent demand) is found in the utilization of composts. Supposing the actual demand ratio is 50%, research again analyzes the demand-supply balance, revealing that all livestock manure composts can be utilized, but that straw compost is surplus in large amounts in the studied regions.
Continuous cropping of cotton causes accumulation of allelochemicals in soil that results in substantial crop yield and quality losses. To elucidate the physiological mechanism of the effects of allelochemicals on cotton root growth, and solve the problem of continuous cropping obstacles, hydroponics experiments were carried out to study the effects of three allelochemicals (p-hydroxybenzoic acid (PHBA), phloroglucinol, and ferulic acid) at different concentrations (0.8, 4.0, and 20.0 mmol L⁻¹) on the production of reactive oxygen species, antioxidant enzyme activities, and mitochondrial function of cotton seedling roots. All three phenolic compounds suppressed cotton root growth, decreased the activity of antioxidant enzymes (superoxide dismutase, catalase and peroxidase) and H⁺-ATPase in root mitochondria, and increased generation of O₂⁻ and the content of H₂O₂. They also increased the degree of openness of mitochondria permeability transition pores, and decreased the membrane fluidity of mitochondria, and the ratio of cytochrome (Cyt) c/a, thus resulting in the damage of mitochondrial structure and overall function of the root system. Ferulic acid at 20.0 mmol L⁻¹ inhibited cotton root growth more than the other treatments. Above all, all three kinds of allelochemicals inhibited antioxidant enzyme activity and mitochondrial function in cotton seedling roots, and the inhibition depended on the dose of phenolic compounds. Compared to PHBA and phloroglucinol, ferulic acid was a stronger inhibitor of cotton seedling root growth.
Estimating field crop evapotranspiration (ETc) along with its components of soil evaporation and crop transpiration is crucial for developing effective agricultural water-saving strategies. This study separately estimated ETc and its components in cotton fields using a SIMDualKc dual crop coefficient model. Data from a 2-year experiment (2010-2011) were used for model calibration and validation analysis. The experimental plots were irrigated at 1.00, 0.85, 0.70, 0.55, and 0.45 of full irrigation amount in north Xinjiang, China. Based on the results, SIMDualKc accurately estimated actual evapotranspiration (ETc adj) and its components under deficit drip irrigation conditions. Cotton field transpiration accounted for 85.4-94.3% of the ETc in the study area. Although plastic film significantly limited evaporative water loss, the fraction of water loss was still 5.7-14.6% of irrigation water. The adopted estimation method could lead to better understanding of where and when water loss occurs and how much water should be used for maximal benefit. A better understanding of these factors is critical for sustainable agricultural water management practices.
The constitutive expressor of pathogenesisrelated genes 5 (CPR5) plays a role in pathogen defence responses, programmed cell death, cell wall biogenesis, seed generation and senescence regulation in plants. Here, we investigated the functional characteristics of CPR5 to long-term heat stress in Arabidopsis with different genotypes: wild type (WT), cpr5 mutant and cpr5/CPR5 complementary transgenic plant. The cpr5 mutant showed increased susceptibility to long-term heat stress, displaying significant decreases in hypocotyl elongation, seedling and inflorescence survival, membrane integrity and photosystem II activity (Fv/Fm) during heat stress. However, the thermotolerance was recovered when cpr5 mutant was transformed with a CPR5 gene. H₂O₂ accumulation and lipid peroxidation were lower in cpr5/CPR5 plants and WT than in cpr5 mutants after exposure to 36 °C for 5 days. The alleviated oxidative damage was associated with increased activities of superoxide dismutase, catalase, and ascorbate peroxidase. Furthermore, the induced expression of HSP17.6A-CI, HSP101 and HSP70B under long-term heat stress was more substantial in cpr5/CPR5 plants and WT than in cpr5 mutants. These findings suggest that CPR5 plays an important role in thermotolerance of Arabidopsis by regulating the activities of antioxidant enzymes and the expressions of heat shock protein genes.
Schima superba and Pinus massoniana distributed over large areas in southern China both are dominant species at Dinghushan Biosphere Reserve. In the present study, the changes of chlorophyll fluorescence and xanthophyll cycle in the leaves of S. superba and P. massoniana exposed to simulated acid rain (SAR) were measured. When exposed to high light, the PSII photochemistry efficiency (Fv/Fm), efficiency of energy conversion in PSII (ΦPSII) and photochemical quenching (qP) of both S. superba and P. massoniana all decreased when acidity of SAR increased. Regarding non-photochemical quenching (qN), S. superba exposed to SAR had higher value than control plants, but there was no significant difference between the respective seedlings of P. massoniana. As for xanthophyll cycle of the two plant species, the leaves of S. superba exposed to SAR showed a higher content of carotenoids and a higher ability to convert violaxanthin to zeaxanthin than leaves of P. massoniana, which was consistent with S. superba exhibiting a stronger resistance to high light than P. massoniana. Although both species were susceptible to acid rain as shown by our results, P. massoniana was more susceptible compared to S. superba. These results provide an insight into how to protect the forest ecosystem at Dinghushan Biosphere Reserve.
Background: Epstein–Barr virus (EBV) infection is causatively associated with a variety of human cancers, including gastric cancer (GC), which has one of the highest mortality rates of all human cancers. Long non-coding RNAs (lncRNAs) show important regulatory roles in human GC. SNHG8 is a recently identified lncRNA that was reported to show abnormal expression pattern in GC. However, little is known of its biological function in EBV-associated GC. Methods: We used cell viability, colony formation and cell cycle assays to investigate the roles of lncRNA SNHG8 in the cell growth of EBV-associated GC. Results: The transcript levels of SNHG8 in the cultured EBV-associated GC cells were significantly higher in the cultured EBV-associated GC cells compared with the levels in normal human gastric mucosal cells and EBV-negative GC cells. Knockdown of SNHG8 with specific shRNAs inhibited cell proliferation and colony formation and arrested the cell cycle in the G0/G1 phase in vitro. We also found that knockdown of SNHG8 suppressed tumor growth in vivo. Conclusions: These data indicate the pro-oncogenic potential of SNHG8 in EBVassociated GC, meaning it is a latent therapeutic target for the treatment of this type of cancer
Continuous rainfall during the harvest period of soybean leads to the delayed harvest of mature seeds and favors the infection of field mold that eventually causes seed mildew in the field under high humid conditions. Soybean isoflavones are a subgroup of flavonoids which possess crucial roles in the responses of soybean to multiple abiotic and/or biotic stresses. To fully reveal the role of isoflavones in the responses of soybean seeds to filed mildew (FM) stress, two soybean genotypes with different resistance to FM stress were treated in a controlled greenhouse which provided high humid conditions in this current research. The dynamic changes of isoflavones contents and the expression levels of four major genes involved in isoflavones biosynthesis pathway were measured by liquid chromatography–mass spectrometry (LC–MS) and quantitative real-time PCR (qRT-PCR), respectively. The current results showed that the contents of major isoflavones components in resistant genotype C103 were higher than the susceptive genotype ND12 before FM treatment. Whereas after FM treatment, the dynamic changing patterns of isoflavones contents and some major genes expression were opposite between these two genotypes, and the biosynthesis of isoflavones was more rapid in resistant genotype compared to susceptible one. Noticeably, the biosynthesis of isoflavones aglycones which exhibit better bioactivities was remarkably promoted after FM treatment in resistant genotype C103, further revealing the important role of isoflavones in the resistance against FM stress. In sum, this research investigated the role of isoflavones against FM stress, the results demonstrated higher inherent contents and more rapid biosynthesis of isoflavones after FM treatment could establish stronger resistant foundation in soybean seeds against the FM stress. These results would provide new insights into the mechanism research of soybean against FM stress, but how the stress systematically regulates isoflavones and other resistant network still needs further research.
Low temperature is an important abiotic stress for plant development and has serious effects on crop production. Because tobacco is sensitive to low temperature, it is suitable for analyzing the mechanisms of cold response in plants. In the current study, NC567 and Taiyan8, two cultivars with different sensitivities to low temperature, were used in Isobaric Tags for Relative and Absolute Quantitation (iTRAQ)-based proteomics to uncover their different mechanisms in response to cold stress. A total of 4317 distinct proteins were identified and the differentially expressed proteins in four comparison sets were used for further analysis. The gene ontology (GO) analysis indicated that the majority of differentially expressed proteins were in the categories involved in metabolic and cellular processes. Surprisingly, there were 55 proteins decreased in NC567, but increased in Taiyan8 in response to cold, while the levels of 42 proteins were lower in Taiyan8 than NC567 at normal temperature, but higher in Taiyan8 than NC567 under cold treatment, suggesting different responses to cold stress in these cultivars. The levels of polypeptides involved in protein synthesis and degradation, photosynthesis, and respiration, as well as ROS scavenging, were different in the comparison sets, implying that protein and energy metabolisms may be important for the establishment of cellular environment at low temperature. In conclusion, our study identified the potential pathways involved in low-temperature response of tobacco and provides hints for the further improvement of cold tolerance in crops.
Cassava (Manihot esculenta Crantz) is a tropical and subtropical plant and susceptible to chilling injury. In this research, a C-repeat binding factor (CBF)-like gene (GenBank accession number JQ339740) has been isolated from cassava, and named as MeCBF1. The full-length DNA of MeCBF1 is 1,037 base pair (bp), without intron. The 5' untranslated region is 102 bp, the 3' untranslated region is 239 bp, and the open reading frame is 696 bp encoding 231 amino acids. The deduced amino acid sequence of MeCBF1 contains two CBF conserved motifs of PKK(P/R)AGRxKFxETRHP and DSxWR. The MeCBF1 shows 83 % homology to the CRT/DRE binding factor 1 from Hevea brasiliensis (Accession no. AAY43213.1). However, in cassava, the MeCBF1 target genes showed low similarity to the CBF/DREB regulated genes in Arabidopsis thaliana. Quantitative real-time PCR showed that the MeCBF1 was highly expressed in stems and leaves, and lowly expressed in roots. In addition, the expression of the MeCBF1 quickly responded to low temperature stress (4°C). These results suggest that, the MeCBF1 is functional in cassava. Further studies on the MeCBF1 might be helpful to reveal molecular mechanism of cassava’s high sensitivity to low temperature.
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