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It is a simple but effective measure to ensure food security by planting rice cultivars with low accumulation ability of heavy metals, especially with Cd-exclusive ones. Eighty-nine pairs of soil and 17 main rice cultivars from the slight-moderate Cd-pollution paddy field in Chengdu Plain were randomly collected. The Cd content in brown rice, Cd enrichment, translocation factors, and rice yield were studied for screening rice cultivars with low Cd uptake and accumulation in a slightly moderate Cd-pollution environment. The results showed that cadmium content in different parts of rice varies in a large range. Cadmium content in brown rice ranged from 0.05 mg·kg-1 to 0.48 mg·kg-1, specifically from 0.01 mg·kg-1 to 0.19 mg·kg-1 in chaff. Among the 17 main rice cultivars, the content of Cd in brown rice of Kyou 817 was 0.46 mg·kg-1, which lay in the highest level, while the Cd content in brown rice of Aiyou 82, Jinyou 527, Gangyou 22, Fuyou 130, IIyou 906, Yixiangyou 1577, Jinyou 725, and Fuyou 838 were lower than national maximum level (0.2 mg·kg-1). Cadmium content in brown rice, chaff, and straw of Aiyou 82 were notably lower than the average value. The Cd content in roots of IIyou 906 reached a relatively high level, but the rest is lower than the average. The enrichment factors of straw in Fuyou 130 and Jinyou 725 (EFs>0.6) were relatively higher than the average. The straw of Fuyou 130, Yixiangyou 1577, and Jinyou 725 had a stronger ability of Cd translocation (translocation factors >0.45, TFs), which exceeded the average. The yield of Aiyou 82 was lower than the average in 90% level, which was remarkably low. In conclusion, IIyou 906 was the most ideal rice cultivar by evaluating the Cd content in brown rice, EFs and TFs in straw, and yield levels. As for Fuyou 130, special attention should be paid to reduce the risk of Cd pollution in straw returning. Gangyou 22, Jinyou 527, and Fuyou 838 presented lower yields or lighter Cd-resistance ability aboveground.
The gene encoding solute carrier family 6 member 14 (SLC6A14) has been considered as a candidate gene affecting human obesity. In this study, full-length cDNA (2237 bp) and DNA sequence (24 541 bp) of the porcine SLC6A14 gene were isolated. The porcine SLC6A14 cDNA contains a 5'-untranslated region of 57 bp, a 3' -untranslated region of 254 bp, and an open reading frame of 1926 bp, encoding a deduced protein of 642 amino acids with a molecular mass of 72. 475 kDa and an isoelectric point of 7.82. The genomic structure of the porcine SLC6A14 gene is similar to mammalian orthologs, particularly in terms of exon size and exon/intron boundaries. It comprises 14 exons and 13 introns. A semi-quantitative RT-PCR showed that the porcine SLC6A14 mRNA expression was tissue-specific. Four SLC6A14 single-nucleotide polymorphisms (SNPs) were identified, and 3 informative SNPs were chosen for genotvping in a White Duroc × Erhualian resource population with phenotvpe data of growth and fatness traits. The association analysis showed that the c. 1438 G>A nonsynonymous polymorphism was associated with birth weight and 21-day body weight (P < 0.05), while g.7944 A>T was associated with 46-day body weight. Linkage and radiation hybrid mapping assigned SLC6A14 to a region around SW1522 on SSCXp13, which did not fall in the confidence interval of the quantitative trait locus (QTL) for growth and fatness traits on SSCX in the resource population. These results indicate that SLC6A14 is not a positional candidate gene for the QTL affecting fatness and growth traits in pigs.
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