The occurrence and removal of sanitary indicator bacteria (TC, FC, FS) and bacteria of Enterobacteriaceae family during the standard sewage treatment process were investigated. In addition, we determined the antibiotic-resistance of Enterobacteriaceae bacteria in raw and treated sewage. The result of the conducted study demonstrated that bacteria of Enterobacteriaceae family were most numerous among the surveyed microorganisms, and fecal streptococci was the least numerous bacteria group. The survey of antibiotic resistance of Enterobacteriaceae family demonstrated that these bacteria are the least sensitive to streptomycin and erythromycin. The increase in the proportion of bacterial strains resistance to tested antibiotics in the subsequent months of the study was correlated with the upward seasonal trend in human population morbidity, and in turn the usage antibiotic therapy.