This is the first report of somatic embryogenesis from leaves of two strawberry cultivars (Selva and Comarosa) cultured on MS medium containing picloram. Maximum embryogenesis was induced using 2 mg/l picloram. Globular stage embryos developed into cotyledonary ones after transfer to hormone-free media containing 2%, 4%, 6%, 8% and 10% (w/v) sucrose. Increasing sucrose concentrations in culture media enhanced somatic embryo development. Cotyledonary somatic embryos were converted to plantlets after transfer on MS medium containing GA3, and maximum conversion was achieved with 1 mg/l and 2 mg/l GA3. Plantlets were capable of continuous growth under greenhouse conditions.
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