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The qualitative and quantitative studies, presented in this article, on wood anatomy of various species belonging to ancient Magnolia genus reveal new aspects of phylogenetic relationships between the species and show evolutionary trends, known to increase fitness of conductive tissues in angiosperms. They also provide new examples of phenotypic plasticity in plants. The type of perforation plate in vessel members is one of the most relevant features for taxonomic studies. In Magnolia, until now, two types of perforation plates have been reported: the conservative, scalariform and the specialized, simple one. In this paper, are presented some findings, new to magnolia wood science, like exclusively simple perforation plates in some species or mixed perforation plates – simple and scalariform in one vessel member. Intravascular pitting is another taxonomically important trait of vascular tissue. Interesting transient states between different patterns of pitting in one cell only have been found. This proves great flexibility of mechanisms, which elaborate cell wall structure in maturing tracheary element. The comparison of this data with phylogenetic trees, based on the fossil records and plastid gene expression, clearly shows that there is a link between the type of perforation plate and the degree of evolutionary specialization within Magnolia genus.
The study has been carried out to determine the frequency of C. difficile recovery in stool cultures and the rate of C. difficile toxin A detection in faecal specimens of patients with nosocomial diarrhoea. Clinical specimens comprised 4414 stool samples collected from 1998 to 2002 from adult patients hospitalised in different wards of a university-affiliated hospital (1200 beds) and suspected of C. difficile-associated disease (CDAD). There have been 1308 (29.6%) specimens positive for C. difficile culture (15.1% in 1998, 29.5% in 1999, 33.8% in 2000, 31.2% in 2001 and 32.0% in 2002). The highest number of C. difficile strains was cultured from stool samples of patients hospitalised in the haematology/oncology ward (51.1% of all isolates), neurology (8.3%), nephrology (8.0%), gastrointestinal surgery (7.0%) and neurosurgery (6.2%) wards. The testing for C. difficile toxin A yielded 847 (19.2%) positive samples and 3567 (80.8%) toxin A-negative results. The percentage of C. difficile toxin A-positive samples was 29.4% in 1998, 17.5% in 1999, 23.2% in 2000, 17.1% in 2001 and 15.0% in 2002. In the analysed period we observed an increase in the number of stool specimens tested for C. difficile and an increase in the number of C. difficile culture-positive samples. A decrease in the number of C. difficile toxin A-positive samples was noted in the last 2 years of the study. This phenomenon may be due to an improved antibiotic policy of the hospital.
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