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Completion of rice genome sequencing has necessitated identification of transcripts encoded by the genome and their corresponding functions. In the present study we have catalogued cytoplasmic ribosomal protein complement of rice genome corresponding to 68 families. Mining TIGR (The Institute for Genomic Research) rice database and unigene sequences available from National Centre for Biotechnology Information (NCBI) produced a non-redundant set of these sequences. This resulted in identification of 209 candidate r-proteins of which 22 have not been reported previously. The number of genes per family ranges from 1 to 8 distributed throughout the genome with maximum occurrence at chromosome 7. Mapping of r-proteins on BAC clones revealed several small clusters of genes. Unigene sequences correspondtng to most of the reported r-proteins were identified indicating these genes are being expressed.
Four tobacco lines over-expressing LlaNAC (FJ423495) gene have been assessed for various parameters like abiotic stress response, phytochemical constituents, biomass composition, response to elevated carbon dioxide (CO₂), etc. All the analyses have been carried out in T2 generation of the transgenic plants with proven genetic stability. In response to the heat and drought stress, the accumulation of antioxidant enzymes elevated up to 2.5-fold. Photosynthetic pigments, i.e., Chl a, Chl b and xanthophylls, and phenolics in the transgenic lines were found significantly (two–fourfold) enhanced in comparison to wild-type (WT) plants. The transgenic lines were also shown capturing 5–10 times more CO₂ from the environment, which presumably gets accumulated as biomass. However, ratio of dry weight to fresh weight was slightly in favor of the WTs. Cellulose, hemi-cellulose and lignin contents in stem and leaves of each of the transgenic lines varied, and overlapped with the lignocellulosic contents of the WT plants. In conclusion, LlaNAC gene shows promise for exploitation in design of the future crops with various end uses.
Lepidium latifolium L., a weed distributed in the Ladakh region of Himalayan range, belongs to Brassicaceae family and reported to withstand low temperature stress <-20°C. RACE primers were designed from EST encoding Ras-related GTP-binding like protein (FG618354) from L. latifolium and full-length LlaRan (GU014818) was obtained. Its cDNA sequence consisted of 672 bp long open-reading frame, 5'UTR of 95 bp and 3'UTR of 115 bp, respectively. The predicted Lepidium RAN protein encodes a 223 aa protein of 25.59 kDa and pI 6.08. In silico characterization of LlaRan suggested that it has a universal RAN domain across species and likely to follow similar functions. Transcript accumulation studies in response to cold stress suggested that it is an early down-regulated gene but a late upregulated gene. Quantitative analysis using real-time PCR revealed differential regulation of the transcript not only under cold stress but also under the effect of stress regulators like jasmonic acid, salicylic acid, calcium, abscisic acid and ethylene which suggests a possible crosstalk between different pathways where LlaRan may have an important role to play. Thus, LlaRan is a candidate gene for engineering plants against abiotic stresses after its further functional validation in model plants.
Vicia faba L. (faba bean) is an important legume and is cultivated essentially as a cool-season crop. Changes in sowing dates and lack of precipitation expose faba bean crop to drought and heat stresses. The gradual rise in global temperatures owing to climate change is likely to exacerbate the detrimental effects of hot and dry climatic conditions on faba bean cultivation. High temperature stress is particularly damaging to faba bean during the flowering period, when the viability of pollen is critical for successful reproduction. Recent studies have shown that maintenance of protein homeostasis through synthesis of heat shock proteins plays a key role in the heat response of plants. To date, there has been no significant work linking the heat response of faba bean to the repertoire of its heat shock proteins. While quantitative trait loci have been identified for resistance against biotic stresses in faba bean, there is no parallel success with abiotic stresses in this species. Programs aiming at genetic improvement of the heat/drought resistance of this crop by both conventional breeding and molecular breeding methods are hampered because of the large and majorly ill-analyzed genome of faba bean plants. Likewise, molecular and biotechnology- related tools are poorly developed for faba bean; as a result, the fruits of transgenic research developed with model plant species are not reaching this crop. While specifically discussing the prospects for the genetic improvement of faba bean against heat and drought stresses, we highlight the areas of research which need to be strengthened on faba bean.
We cloned and characterized the full-length coding sequence of a small heat shock protein 17.9 gene from faba bean encoding 160 amino acids and containing the conserved a-crystallin domain at the C-terminus. Homology and phylogenetic analysis suggested its proximity with the class II sHsp members of fabaceae family. Therefore, we name this gene as VfHsp17.9-CII. The VfHsp17.9-CII transcript showed a clear heat stress induction pattern in leaves of young seedlings and flowering plants. Transient expression of VfHsp17.9-CII fused with green fluorescent protein reporter indicated its nuclear localization. Overexpression of recombinant VfHsp17.9- CII protein in Escherichia coli cells increased tolerance of the bacterial cells to heat and arsenic stresses. The reduction of faba bean pollen viability in response to heat stress correlated with the accumulation pattern of VfHsp17.9-CII transcript in heat stressed pollen. It is suggested that VfHsp17.9-CII protein plays a key role in heat and heavy metal stress tolerance.
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