Preferencje help
Widoczny [Schowaj] Abstrakt
Liczba wyników

Znaleziono wyników: 17

Liczba wyników na stronie
Pierwsza strona wyników Pięć stron wyników wstecz Poprzednia strona wyników Strona / 1 Następna strona wyników Pięć stron wyników wprzód Ostatnia strona wyników

Wyniki wyszukiwania

help Sortuj według:

help Ogranicz wyniki do:
Pierwsza strona wyników Pięć stron wyników wstecz Poprzednia strona wyników Strona / 1 Następna strona wyników Pięć stron wyników wprzód Ostatnia strona wyników
Seed dispersal distance is influenced by a variety of seed properties and functional responses of dispersers. However, to our knowledge, how and why seed dispersal distances are determined remains poorly understood. In the present study, seeds of sympatric tree species, Pinus koraiensis, Corylus mandshurica, Corylus heterophylla, and Quercus mongolica were released to investigate the effects of rodent abundance, seed type, and seed availability on seed dispersal. Our results showed that seeds of P. koraiensis were dispersed further than those of C. heterophylla and C. mandshurica regardless of the ambient rodent and seed abundances, reflecting a consistent effect of seed type on seed dispersal distances. Seed dispersal distance was greatly facilitated by lower per-capita seed abundance (the ratio of seeds to rodents); however, seed caching and cache survival were benefited from higher per-capita seed abundance. Although seed dispersal and seed caching of a particular tree species can be enhanced by its own seed availability, no consistent influence was detected at interspecific levels, reflecting different interspecific effects of seed availability on seed dispersal of sympatric seed species. Our results provide evidences that the effect of seed availability on seed dispersal should be evaluated in terms of per-capita seed abundance and interspecific effects, rather than the independent influence of seed or disperser abundances.
We investigated the response of Mn-hyperaccumulator Phytolacca americana L. to manganese excess as well as the relationships between lignin deposition in the plant’s leaves, peroxidase and laccase activities in the leaf apoplast, and Mn toxicity. The exceptionally high tolerance of P. americana to Mn, both in solution and in tissue, was confirmed. No visible brown spot was observed in the leaves of plants treated with B10,000 µM Mn for 10 days. Mn treatment significantly increased lignin content and laccase activity in the apoplastic washing fluid (AWF) of P. americana leaves. In contrast, an increase in the Mn supply was paralleled by a significant decrease in the concentration of total phenolic compounds (TPCs) and in water-soluble guaiacol peroxidase (SPOD) activity in leaf AWF. This result suggested that an increase in lignin deposition decreased the concentration of apoplastic TPCs that are available to generate potentially toxic intermediates by acting as peroxidase substrates. Thus, data of the present study indicate that lignin formation by laccase activities reduces Mn toxicity and increases Mn tolerance of P. americana by depressing SPOD-mediated formation of toxic intermediates from TPCs.
To shed light on the relationship between sucrose metabolism and expression of genes related to sucrose-metabolizing enzymes, six genes encoding sucrose-metabolizing enzymes were isolated, and the levels of four main carbohydrates and related enzyme activities as well as the expression of these six genes were determined in fruits, leaves and phloem-enriched fraction throughout peach fruit development. Sucrose content in mature fruit ranked first followed by glucose, fructose and sorbitol in that order, while sorbitol was the highest and sucrose lowest in phloem-enriched fraction and leaves. Glucose and fructose had similar change patterns throughout fruit development. Cloning results reveal that the nucleotide sequences of the six genes have high similarity to corresponding genes isolated from other plants. In addition, the expression of these genes and the levels of related enzyme activities varied with tissue and stage of fruit development, suggesting a complexity in relationships between carbohydrates, enzymes activities and related gene expression. Sucrose phosphate synthase maybe a key enzyme involved in sucrose synthesis while sucrose synthase may mainly be responsible for sucrose synthesis in peach fruits at later stages of development. Further studies are needed to genetically and physiologically characterize these genes and enzymes in peach and to gain a better understanding of their functions and relationship with carbohydrate metabolism.
Sucrose synthase (SUS) has been suggested to play a key role in plant sucrose metabolism with recent studies reporting that a small number of genes encoding different isozymes of Sus exist in most plant species. Despite this, information on genes encoding different isozymes of Sus in peach (Prunus persica) is scanty. In this study, we report the prediction, isolation, structural characteristics, phylogenetic connections and expression outline of six Sus genes in peach (PpSus1 to 6). The six PpSus genes were found distributed across scaffolds 1, 3, 5, 7, and 8. Analysis of the exons/introns revealed that PpSus genes contain multiple introns that range from 11 to 13 and displayed a high degree of conservation with corresponding Sus genes in other plant species. The comparative screening of motifs in PpSus proteins indicated high conservation in terms of number, width and order of motifs among PpSus proteins, which indirectly indicates that the six PpSus proteins are indeed members of the SUS family. Phylogenetic analysis revealed that PpSus2 to PpSus4 belonged to group II of the Sus family, PpSus5 and PpSus6 were clustered into group III, and group I contained only one peach gene (PpSus1) together with members from 10 other plant species. Analysis of expression levels of the six PpSus genes revealed that transcripts of PpSus1 were almost undetectable in leaves and in older phloem, while PpSus2 and PpSus4 were almost undetectable in flowers. The other three PpSus genes appeared differentially expressed in all tissues examined and were detected at different stages of tissue development. The results obtained from this study will be useful in selecting candidate PpSus genes for further functional analysis in the pathway of sucrose metabolism in peach and specifically in characterizing the knockout/knockdown mutants of PpSus genes.
This study aims to investigate the effects of oxytetracycline on growth and photosynthetic capacity of rape (Brassica campestris L.). Four levels of oxytetracycline (0, 10, 100, and 200 mg kg⁻¹) were added to the potted soil. Chlorophyll fluorescence parameters induced by five levels (90, 190, 420, 820, and 1,500 μmol photons m⁻² s⁻¹) of PAR (PAR, photosynthetically active radiation) were measured. Plant growth indices and leaf traits were also determined. Electron transport rate increased along with the increase of PAR, but an opposite trend was found for the effective quantum yield of PSII. The quantum yield of light-induced non-photochemical fluorescence quenching in higher oxytetracycline treatments (100 and 200 mg kg⁻¹) gradually increased when PAR increased from 90 to 820 μmol photons m⁻² s⁻¹, but then declined under higher PAR gradients. The fractions of quantum yield of non-light-induced non-photochemical fluorescence quenching in PSII were significantly higher in all the oxytetracycline treatments than in the control. Oxytetracycline exposure was also found to alter the energy distribution in the photosynthetic electron transport chain. This study showed that oxytetracycline exposure evidently diminished the photosynthetic capacity of rape, which was further supported by the observations that growth indices and leaf traits were significantly inhibited by oxytetracycline.
EhMT1, a type 1 metallothionein (MT) gene, was cloned from Elsholtzia haichowensis, a Cu-tolerant plant. Typical of plant type 1MTs,EhMT1encodes a putative peptide of 74 amino acid residues containing cysteine-rich domains. Sequence comparisons with various databases revealed strong similarities at the nucleotide and amino acid levels between EhMT1 and the type 1 MT of Mimulus guttatus. EhMT1 transcriptionwas greater in the roots than in the leaves, and was markedly increased by treatments with Cu, H₂O₂, and heat shock. EhMT1-GUS was localized to the cytoplasm of onion epidermal cells. Escherichia coli cells expressing pET-30a-EhMT1 were more tolerant to Cu and accumulated more Cu than control cells. Our results show that EhMT1 is involved in Cu tolerance and accumulation in E. haichowensis.
As a member of tyrosinase-related family, tyrosinase-related protein-1 (TYRP1) has been reported to play an important role in melanin biosynthetic pathway in some species, but little is known about its role in goat. In this study, almost complete goat TYRP1 gene (17554 bp, HMO70243) encompassing complete CDS was identified, the coding region amounting to 1614 bp, distributed in 7 exons (2-8), and intron 5 was found to possess GC-AG as its splice sites. Dozens of SNPs as well as simple sequence repeat (SSR) motifs were detected in goat TYRP1 gene, in which 4 SSR motifs in intron 5 are combined in a tandem. Correlation analysis showed the allele A to be advantageous compared to C at site g.1263A>C and allele C compared to T at g.1428C>T, implyimg that haplotype AC is in favour of eumelanin biosynthesis, and haplotype CC is in favour of pheomelanin formation.Marked deficiency of heterozygotes occurred in Nanjiang Yellow Goat Black strain and Nanjiang Yellow Goat Fast Grow strain, indicating the high inbreeding in both strains.
The rice (Oryza sativa L.) elongated uppermost internode (eui) gene effectively results in a recessive tall plant type. In eui background, we found a mutant 02428ha that exhibited a semidwarf phenotype. Because semidwarf varieties possess high yield potential, this mutant may be useful for hybrid rice breeding. The present study aimed to characterize the dwarfing mechanism in the mutant. Microscopic observation revealed that the cell length in the uppermost internode was reduced compared to the wild type. The mutant showed a decreased sensitivity to exogenous gibberellin A₃ (GA₃) and gibberellin (GA) biosynthesis inhibitor, uniconazole. Meanwhile, it contained a high level of endogenous gibberellin A₁ (GA₁). These results indicated that the semidwarf phenotype of 02428ha was caused by impairment of GA signal transduction rather than by block in GA biosynthesis. Furthermore, expression analysis of genes involved in the GA signaling pathway showed that transcript of GIBBERELLIN INSENSITIVE DWARF2 (GID2) was down-regulated in the mutant. Sequence analysis also revealed a 15-base pair (bp) insertion between base 594 and 595 in the coding region of GID2. Consequently, the reason for down-regulation expression of GID2 or sequence variation of GID2 might play roles in the phenotype of 02428ha.
Pierwsza strona wyników Pięć stron wyników wstecz Poprzednia strona wyników Strona / 1 Następna strona wyników Pięć stron wyników wprzód Ostatnia strona wyników
JavaScript jest wyłączony w Twojej przeglądarce internetowej. Włącz go, a następnie odśwież stronę, aby móc w pełni z niej korzystać.