Moss and lichen bag technique with use of three mosses (Pleurosium spp., Rhytidiadelphus spp., Polytrichum spp.) and one lichen Pseudevernia furfuracea taxon was used to monitor air quality in underground parking lot in the city of Prešov, Slovakia. Moss and lichen bags were exposed for 6 weeks at different distances from the entrances/exits. Accumulation ability was expressed by relative accumulation factor. The study aims to detect the level of pollution by the risk elements using relative accumulation factor (RAF) and compare accumulation abilities of different moss and lichen taxa. Accumulation ability of exposed taxa decreased in order: Pleurosium spp. > Rhytidiadelphus spp. > Pseudevernia furfuracea > Polytrichum spp. Compa-ring the evaluated elements, Zn and Ni reached the highest RAF values. The distance from the entrance/exit did not affect the level of elements. It is not being given much attention to the air quality assessment in enclosed parking lots in the world, and this is the first such kind of research in Slovakia