Wyniki wyszukiwania

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Wpływ dodatku węgla brunatnego i torfu na bioutylizację odpadów tłuszczowych - wstępne badania laboratoryjne

100%

Wierzba S., Latala A., Latala B.

Biotechnologia

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2000

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nr 3

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Biological utilisation of fatty waste - initial laboratory examination

100%

Latala A., Wierzba S., Latala B.

Biotechnologia

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2000

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nr 1

3

The Escherichia coli RNA polymerase alpha subunit and transcriptional activation by bacteriophage lambda CII protein

76%

Gabig M., Obuchowski M., Ciesielska A., Latala B., Wegrzyn A., Thomas M. S., Wegrzyn G.

Acta Biochimica Polonica

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1998

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tom 45

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nr 1

Bacteriophage λ is not able to lysogenise the Escherichia coli rpoA341 mutant. This mutation causes a single amino acid substitution Lys271Glu in the C-terminal domain of the RNA polymerase α subunit (αCTD). Our previous studies indicated that the impaired lysogenisation of the rpoA341 host is due to a defect in transcriptional activation by the phage CII protein and suggested a role for αCTD in this process. Here we used a series of truncation and point mutants in the rpoA gene placed on a plasmid to investigate the process of transcriptional activation by the CII gene product. Our results indicate that amino-acid residues 265, 268 and 271 in the α subunit may play an important role in the CII-mediated activation of the pE promoter (most probably residue 271) or may be involved in putative interactions between αCTD and an UP-like element near pE (most probably residues 265 and 268). Measurement of the activity of pE-lacZ, pI-lacZ and paQ-lacZ fusions in the rpoA+ and rpoA341 hosts demonstrated that the mechanism of activation of these CII-dependent promoters may be in each case different.

Ograniczanie wyników

2 Biotechnologia

1 Acta Biochimica Polonica

3 Latala B.

2 Latala A.

2 Wierzba S.

1 Ciesielska A.

1 Gabig M.

1 Obuchowski M.

1 Thomas M. S.

1 Wegrzyn A.

1 Wegrzyn G.

2 2000

1 1998

Wpływ dodatku węgla brunatnego i torfu na bioutylizację odpadów tłuszczowych - wstępne badania laboratoryjne

Biological utilisation of fatty waste - initial laboratory examination

The Escherichia coli RNA polymerase alpha subunit and transcriptional activation by bacteriophage lambda CII protein