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1

Zespół zaburzeń alimentarnych u prosiąt, warchlaków i tuczników - nowe dane dotyczące Brachyspira hyodysenteriae, Lawsonia intracellularis, Clostridium spp. oraz enteropatogennych szczepów E. coli

100%
Zmudzki J., Dors A., Nowak A., Grzesiak A.
Lecznica Dużych Zwierząt. Ogólnopolski Kwartalnik dla Lekarzy Weterynarii
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2013
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tom [08]
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nr Monografia Wyd. Spec.
2

Factors affecting the freezing point of milk from Polish Holstein-Friesian cows

100%
Otwinowska-Mindur A., Ptak E., Grzesiak A.
Annals of Animal Science
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2017
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tom 17
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nr 3
3
Artykuł dostępny w postaci pełnego tekstu - kliknij by otworzyć plik
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Współpraca pielęgniarek szkolnych z nauczycielami wychowania fizycznego w zakresie promocji zdrowia i edukacji zdrowotnej

100%
Bogusz R., Charzynska-Gula M. L., Grzesiak A., Galeziowska E.
Medycyna Ogólna i Nauki o Zdrowiu
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2013
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tom 19(48)
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nr 4
4

Effect of naphtalene-1-acetic acid on growth of the container-produced ornamental shrubs and development of their root system

100%
Pacholczak A., Szydlo W., Grzesiak A.
Annals of Warsaw University of Life Sciences - SGGW. Horticulture and Landscape Architecture
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2009
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tom 30
5

The level of endogenous DNA damage in lymphocytes isolated from blood is associated with the fluctuation of 17beta-estradiol concentration in the follicular phase of healthy young women

100%
Kapiszewska M., Kalemba M., Grzesiak A., Kocemba K.
Acta Biochimica Polonica
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2005
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tom 52
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nr 2
The aim of this study was to evaluate whether the differences in plasma 17β-estradiol concentration in early and late follicular phases of the menstrual cycle can affect the level of endogenous DNA damage in lymphocytes assessed by comet assay, and whether the extent of this damage in the follicular phase is associated with the genotype of catechol-O-methyltransferase (COMT). The level of DNA damage was positively correlated with 17β-estradiol concentration only in the late follicular phase. Subjects with the COMT L/L homozygous mutated variant revealed more DNA damage as compared to individuals with the COMT wild-type and heterozygous (H/L+HH) genotype.
6
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Antimicrobial susceptibility of Brachyspira hyodysenteriae isolated from 21 Polish farms

76%
Zmudzki J., Szczotka A., Nowak A., Strzelecka H., Grzesiak A., Pejsak Z.
Polish Journal of Veterinary Sciences
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2012
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tom 15
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nr 2
Swine dysentery (SD) is a common disease among pigs worldwide, which contributes to major production losses. Antimicrobial susceptibility testing of B. hyodysenteriae, the etiological agent of SD, is mainly performed by the agar dilution method. This method has certain limitations due to difficulties in interpretation of results. The aim of this study was the analysis of antimicrobial susceptibility of Brachyspira hyodysenteriae (B. hyodysenteriae) Polish field isolates by broth microdilution procedure. The study was performed on 21 isolates of B. hyodysenteriae, collected between January 2006 to December 2010 from cases of swine dysentery. VetMIC™ Brachyspira panels with antimicrobial agents (tiamulin, valnemulin, doxycycline, lincomycin, tylosin and ampicillin) were used for susceptibility testing of B. hyodysenteriae. The minimal inhibitory concentration (MIC) was determined by the broth dilution procedure. The lowest antimicrobial activity was demonstrated for tylosin and lincomycin, with inhibition of bacterial growth using concentrations >128 μg/ml and 32 μg/ml, respectively. In the case of doxycycline, the MIC values were ≤ 2.0 μg/ml. No decreased susceptibility to tiamulin was found among the Polish isolates and MIC values for this antibiotic did not exceed 1.0 μg/ml. The results of the present study confirmed that Polish B. hyodysenteriae isolates were susceptible to the main antibiotics (tiamulin and valnemulin) used in treatment of swine dysentery. Further studies are necessary to evaluate a possible slow decrease in susceptibility to tiamulin and valnemulin of B. hyodysenteriae strains in Poland.
7

Lekowrażliwość wybranych krajowych szczepów bakteryjnych wyizolowanych od chorych i padłych świń w latach 2004 - 2010

76%
Zmudzki J., Grzesiak A., Nowak A., Urbaniak K., Stepniewska K., Markowska-Daniel I., Pejsak Z.
Lecznica Dużych Zwierząt. Ogólnopolski Kwartalnik dla Lekarzy Weterynarii
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2012
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tom 07
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nr 1
8
Artykuł dostępny w postaci pełnego tekstu - kliknij by otworzyć plik
Content available

Application of real-time PCR for detection of Lawsonia intracellularis and Brachyspira hyodysenteriage in fecal samples from pigs

76%
Zmudzki J., Szczotka A., Podgorska K., Nowak A., Grzesiak A., Dors A., Pejsak Z.
Polish Journal of Veterinary Sciences
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2012
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tom 15
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nr 2
The aim of the study was to develop and validate real-time PCR method for the quantification of Lawsonia intracellularis and Brachyspira hyodysenteriae in porcine feces. Before the optimization process was performed two different extraction methods were compared to select the more efficient one. Based on the results achieved at this stage the boiling procedure was rejected and a commercially available silica-membrane based method was chosen for further analysis. The primers and the Taqman probe for B. hyodysenteriae and L. intracellularis were based on the sequence of NADH oxidase gene and 16S rDNA gene, respectively. The detection limit of the real-time PCR for suspension of feces inoculated with B. hyodysenteriae and L. intracellularis was determined to be 1.5x103 CFU/ml and 6.5x101 CFU/ml, respectively. The results of this study demonstrate that our real-time PCR is able to detect low number of B. hyodysenteriae and L. intracellularis cells which is satisfying in routine diagnosis of swine dysentery and proliferative enteropathy. Therefore, it is possible to identify both subclinically infected pigs and those representing an acute form of mentioned diseases. In summary, the quantitative real-time PCR is useful for routine diagnosis of L. intracellularis and B. hyodysenteriae. Compared to conventional PCR, the new validated quantification method based on real-time PCR is fast and with reduced risk of laboratory contamination. The novel technique is specific and even more sensitive than the previously used one. Furthermore, the new real-time PCR enables quick detection and quantification of both pathogens in fecal samples, which helps to estimate the health status of a pig herd.
9

Fizjologiczne i genetyczne markery odporności pszenicy jarej na suszę w aspekcie interakcji zachodzących w ryzosferze

52%
Szechynska-Hebda M., Hordynska N., Grzesiak A., Grzesiak M., Sas-Paszt S., Lisek A., Sumorok B., Derkowska E., Weszczak K., Frac M., Przybyl M., Trzcinski P., Gluszek S.
Biuletyn Instytutu Hodowli i Aklimatyzacji Roślin
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2020
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nr 291 suplement
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