The influence of different oxygen and nitrogen concentrations on the speed of waking up after CO2 anesthesia and on the time of starting egg-laying by the artificially inseminated queen bees. This work was carried out in the Bee Division of Warsaw University of Life Science in the 2006 bee season. The purpose of this research was to find factor that speeds up egg-laying by the artificially inseminated queen bees (Apis mellifera carnica L). In the research 82 queen bees were used which were inseminated with a single semen dose of 8 mm³ . All queen bees were anaesthetized with CO2 . Three groups where considered: 1) control - bees were waken up in the atmosphere, 2) - bees were waken up in the air which contained 15% O2 and 85% N2 , 3) - bees were waken up from anesthesia in the air with 35% O2 and 65% N2 . Change of the O2 content in air, which was used for waking up queen bees from anesthesia, caused faster waking up (on average about 8 minutes). However, the change of O2 content in air didn't cause significant changes in the time of egg-laying in comparison to control group.