The life cycle of Acanthostomum burminis (Bhalerao, 1926) Bhalerao, 1936 (Trematoda, Acanthostomidae) infecting Xenochrophis piscator has been elucidated. Life cycle stages were successfully reared in the laboratory. The life cycle, from cercaria to adult, took more than two months to complete. Pleurolophocercous cercariae were found in the thiarid snail Thiara tuberculata. Metacercariae occurred in fin rays of several species of freshwater fishes. Snakes became infected when fed on fishes containing metacercariae. The prepatent period is 28 days. Successive stages in the development of metacercariae and adults were followed.
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