The field experiments with spring barley cultivar Justina were conducted in 2010–2011. The tested products (Regalis 10 WG, Modus 250 EC, Antywylegacz Płynny 675 SL, Cerone 480 SL) were applied on spring barley at the stage of BBCH 32. The height reduction of spring barley was dependent on the active substance of growth regulators and weather conditions, and it was 0–5.9% (2010) and 1.6–9.8% (2011), respectively. Higher effectiveness of shortening stems of spring barley was obtained after the use of Cerone 480 SL at the dose of 0.75 l/ha. In two years of investigation lack of statistic differences between growth retardants prohexadione-Ca, chloromequat chloride and ethephone regardless of pH spray liquid. Lower pH of spray liquid containing trinexapak ethyl showed better efficacy (shorter stems and lower lodging) in comparison to plots on which trinexapak ethyl on neutral pH was used. The examined growth regulators did not cause any injury to spring barley cultivar Justina. Acidity of spray liquid containing growth regulators increased the yield of spring barley in comparison to untreated plots and plots on which growth regulators were used in neutral pH.