In the presented work, we compared the results of determination of rabies antibodies using three in vitro methods: rapid fluorescent focus inhibition test (RFFIT), fluorescent antibody virus neutralisation test (FAVNT) and the immunoenzymatic assay (ELISA). 196 dog sera samples were examined with FAVNT, RFFIT methods and the ELISA test. Sera with low and sufficiently high titre of antibodies had a similar result in determining by all methods. A critical level of rabies antibodies close to the required protection level (0.5 IU/cm3) was seen in sera of 18 dogs (9.18%); these were the sera obtained after primary vaccination of dogs. At this level, even small differences can cause a change in the assessment of the patient's serum seronegativity or seropositivity. Therefore, it is important to choose the appropriate method that has sufficiently strict criteria while having a good reproducibility.
Microbiological screening tests for the presence of E. coli O157:H7 were carried out on 196 samples of raw cow’s milk from different dairies in the East-Slovak region in the period of May-July 2006. Of the total number of examined samples, E. coli strains were isolated from two milk samples (1.02%) from dairies of the Trebišov district. In spite their relatively low incidence, the health consequences of the presence of this pathogen are so important that regular screening of raw milk seems to be necessary to determine the source and way of contamination as well as to make proposal for effective eliminative and precautionary measures.