Wyniki wyszukiwania

Sortuj według:

Ogranicz wyniki do:

Artykuł dostępny w postaci pełnego tekstu - kliknij by otworzyć plik

High frequency of doubled haploid plant production in spelt wheat

100%

Lantos C., Jenes B., Bona L., Cserhati M., Pauk J.

Acta Biologica Cracoviensia. Series Botanica

2016

tom 58

nr 2

This is the first study to report an efficient anther culture (AC) method for spelt wheat, which has an increasing importance not only in applied research but also in organic farming and changing nutritional standards. In this study, an efficient AC protocol has been described for ‘GK Fehér’ spelt wheat. The number of AC-derived embryolike structures (ELS) was 62.2/100 anthers, from which we were able to regenerate 30.6 green plantlets per 100 anthers. The percentage of green plantlets production was 89.0% among the regenerated plantlets, while the phenomenon of albinism was restricted (3.8/100 anthers). Altogether, from AC of ‘GK Fehér’ 306 green plantlets were produced in vitro and 241 plants were acclimatized to the greenhouse conditions. Based on ploidy level analyses, 83 spontaneous doubled haploid (DH) plants were produced (8.3 DH plants/100 anthers), so the percentage of spontaneous rediploidization was 34.4%. The spontaneous DH plants produced fertile spikes, while a few seeds were harvested from seven partially fertile plants.

Ograniczanie wyników

1 Acta Biologica Cracoviensia. Series Botanica

1 Bona L.

1 Cserhati M.

1 Jenes B.

1 Lantos C.

1 Pauk J.

1 2016

Wyniki wyszukiwania

High frequency of doubled haploid plant production in spelt wheat