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Budowa mózgu muchówek z rodziny Tabanidae

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The paper deals with the histological structure of the brain in the specimens of the Tabanidae family including Tabanus, Chrysops and Chrysosona genera. Measurements of the brain volume and of the brain cen tres were made and the ratio of the size of the centres to that of the brain was given on the example of the female of Tabanus bromius. Lobi olfactorii are the best developed brain centres in Tabanidae. They take up 88.53% of the whole brain in the female of Tabanus bromius. Corpora pedunculata are poorly developed (0.14%) and built up of single, small calyces and thin stalks. The central body is well developed and consists of three parts. The central body takes up 0.15% of the size of the brain in the female of Tabanus bromius. Although lobi olfactorii are small when compared with the size of the brain (0.29%), the size of the antennal glomerules and thick fibres of the antenno-globular tracts are an evidence of a well developed sense of smell in those parasites.
Proteins were extracted from F. hepatica collected on cattle and sheep. The extract was purified in the column with sephadex G-25. The proteins were separated using the Davis method in electrophoretic systems constructed by the authors, in the columns and in blocks in polyacrylamid gel. The electrophoregrams served for making densometric diagrams and for calculating electrophoretic mobility coefficient (Rf) of the identified protein bands. Both in the blocks and in the columns 26 protein bands have been identified in F. hepatica from both cattle and sheep. Statistical analysis with t Student test showed significant differences in their Rf values. A high reproducibility of the results has been obtained both in the columns and in the blocks of polyacrilamid gel.
The studies revealed that the infection with the „limax" group amoebae resulted in the severe changes in the respiratory system: in interstitial pneumonia, the injuries of the bronchi, the bronchioles and the blood vessels. The histopathological examinations showed the focal destruction of the alveoli in case of the mild amoeba invasion while the massive invasion caused the complete destruction of the respiratory epithelium, the blood vessels, the bronchi and the bronchioles. Apart from all the changes in the alveoli, in most cases the significant pulmonary hyperaemia and the numerous blood foci were observed. Moreover, the histopathological changes in the blood vessels, the bronchi and the bronchioles consisting in the epithelial destruction and the laceration of the blood vessel walls and the bronchioles were disclosed.
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