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ZNF300, a recently identified human transcription factor, activates the human IL-2Rbeta promoter through the overlapping ZNF300/EGR1 binding site

100%

Xue L., Qiu H., Ma J., Guo M., Li W.

Cellular and Molecular Biology Letters

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2010

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tom 15

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nr 4

ZNF300 was recently identified as a member of the human KRAB/C2H2 zinc finger protein family. Little is known about the role of ZNF300 in human gene regulation networks. In this study, the DNA-binding property of ZNF300 was further analyzed. We found that the recombinant ZNF300 could bind to the binding site 5′-GCGGGGGCG-3′ of Egr1, another member of the KRAB/C2H2 zinc finger protein family. Similarly, recombinant Egr1 also showed a similar binding affinity to the ZNF300 binding site 5′-CTGGGGGCG-3′. Bioinformatics analysis revealed that there is an overlapping ZNF300/Egr1 binding site in the human IL-2Rβ promoter region, which was previously known to be recognized by endogenous Egr1. Electrophoretic mobility shift assays showed that endogenous ZNF300 could also bind to this site. A transient transfection assay revealed that both ZNF300 and Egr1 could transactivate the IL-2Rβ promoter, and that the activation was abrogated by a mutation of residues in the overlapping ZNF300/Egr1 binding site. Co-expression of ZNF300 and Egr1 led to enhanced IL-2Rβ promoter activity. Thus, ZNF300 is likely to be another regulator of the human IL-2Rβ promoter.

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Identification of the DNA binding element of the human ZNF300 protein

86%

Qiu H., Xue L., Gao L., Shao H., Wang D., Guo M., Li W.

Cellular and Molecular Biology Letters

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2008

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tom 13

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nr 3

391-403

The human ZNF300 gene is a member of the KRAB/C2H2 zinc finger gene family, the members of which are known to be involved in various developmental and pathological processes. Here, we show that the ZNF300 gene encodes a 68-kDa nuclear protein that binds DNA in a sequence-specific manner. The ZNF300 DNA binding site, C(t/a)GGGGG(c/g)G, was defined via a random oligonucleotide selection assay, and the DNA binding site was further confirmed by electrophoretic mobility shift assays. A potential ZNF300 binding site was found in the promoter region of the human IL-2Rβ gene. The results of electrophoretic mobility shift assays indicated that ZNF300 bound to the ZNF300 binding site in the IL-2Rβ promoter in vitro. Transient co-transfection assays showed that ZNF300 could activate the IL-2Rβ promoter, and that the activation was abrogated by the mutation of residues in the ZNF300 binding site. Identifying the DNA binding site and characterizing the transcriptional regulation property of ZNF300 would provide critical insights into its potential as a transcriptional regulator.

3

Astragalus polysaccharide protects formaldehyde-induced toxicity by promoting NER pathway in bone marrow mesenchymal stem cells

72%

She Y., Zhao X., Wu P., Xue L., Liu Z., Zhu M., Yang J., Li Y.

Folia Histochemica et Cytobiologica

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2021

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tom 59

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nr 2

4

Protein complex and proteomic profile in the roots of Oryza sativa L. in response to cadmium toxicity

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Lin S., Wang W., Tian T., Wang Y., Liu Q., Zhang W., Ai Y., Xue L., He H.

Acta Physiologiae Plantarum

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2015

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tom 37

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nr 09

Cadmium (Cd), a non-essential and toxic heavy metal element, is extremely harmful for rice growth, food safety and even public health. In this paper, two rice varieties, Shanyou 63 (Tolerant to Cd stress) and Nipponbare (Susceptible to Cd stress), were employed as materials to investigate the protein expression and protein–protein interaction in rice in response to different stages of Cd stress. The result showed that Cd accumulated in the root of both rice cultivars, but the Cd content in the root of Shanyou 63 was significantly lower than that in Nipponbare. Eight proteins were up-regulated in the two rice cultivars, but the expressing abundance of these eight proteins in Shanyou 63 was statistically higher than that in Nipponbare, indicating that the relative abundance of these eight proteins was positively related to the ability of rice cultivars to avoid Cd stress. According to Gene Ontology annotation, four of these eight proteins were involved in energy metabolism, while four participated in stress tolerance. Native-PAGE and LC–MS/MS methods revealed that four GSTs, one cysteine synthase and one protein disulfide isomerase formed a stable protein complex in Shanyou 63 root in response to 3-, 5- and 7-day Cd stresses, but this protein complex did not appear in Nipponbare root under Cd stress. Owing to the same domain, the four glutathione S-transferases (GSTs) and cysteine synthase had the potential to interact and compose a protein complex to detoxify Cd in Shanyou 63 rice plants. Our results provide new insight into the detoxification mechanism of rice plant in response to Cd stress and may prove to be useful for the future research.

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Distribution and expression of SLC45A2 in the skin of sheep with different coat colors

72%

Wang H., Xue L., Li Y., Zhao B., Chen T., Liu Y., Chang L., Wang J.