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Comparison of proanthocyanidins content in Rhodiola kirilowii and Rhodiola rosea roots - application of UPLC-MS/MS method

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Gryszczynska A., Krajewska-Patan A., Buchwald W., Czerny B., Mielcarek S., Rudzinska K., Mroziewicz P. M.

Herba Polonica

2012

tom 58

nr 3

The purpose of presented study was the comparison of flavan-3-ol content in the roots of Rhodiola kirilowii and Rhodiola rosea with use of UPLC-MS/MS method. Two kinds of extract were prepared: aqueous extract and 50% v/v ethanol extract. The worked out UPLC MS/MS method allowed to determine the content of five flavan-3-ols: (+)-catechin, (-)-epicatechin, (-)-epigallocatechin, (-)-epicatechin gallate (ECG) and (-)-epigallocatechin gallate (EGCG). The obtained results shown that the content of measured catechins was higher in R. kirilowii roots than in R. rosea. Both Rhodiola roots contain EGCG as a main proanthocyanidin compound – the content in R. kirilowii roots is about 0.14%. Our results indicate that application of ultra performance liquid chromatograph connected to a tandem mass spectrometer (UPLC MS/MS method) allows to determine the proanthocyanidins content in tested samples with satisfactory precision and can be used in the Rhodiola sp. phytochemical investigations.

Ograniczanie wyników

1 Herba Polonica

1 Buchwald W.

1 Czerny B.

1 Gryszczynska A.

1 Krajewska-Patan A.

1 Mielcarek S.

1 Mroziewicz P. M.

1 Rudzinska K.

1 2012

Wyniki wyszukiwania

Comparison of proanthocyanidins content in Rhodiola kirilowii and Rhodiola rosea roots - application of UPLC-MS/MS method