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Antimicrobial and antioxidant activity of Epimedium pinnatum

100%
Mahboubi M., Kazempour N., Hosseini H., Mahboubi M.
Herba Polonica
|
2013
|
tom 59
|
nr 2
Epimedium pinnatum (Berberidaceae family) is used as an aphrodisiac in traditional Chinese medicine (TCM). The aim of this study was to evaluate the antimicrobial and antioxidant activity of E. pinnatum extracts (ethanol, methanol and aqueous extracts). Total phenolic (TPC) and flavonoid contents (TFC) of each extract were assessed by spectrophotometric methods. It was exhibited that methanol extract had better antimicrobial activity than those of ethanolic extract or aqueous extract. The TPC and TFC of E. pinnatum extracts was higher in methanol extract (149 and 36.6 mg/g) than that of ethanolic extract (137.2 and 19.5 mg/g) and aqueous extract (86.2 and 8.4 mg/g). The methanol extract had lower IC50 value (200 µg/ml) than ethanolic (250 µg/ml) and aqueous extract (400 µg/ml). There was a positive correlation between TPC, TFC in E. pinnatum extract and their antioxidant and antimicrobial activity.
2
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The antimicrobial activity of Prunella vulgaris extracts

100%
Mahboubi M., Mahboubi A., Kazempour N.
Herba Polonica
|
2015
|
tom 61
|
nr 1
Prunella vulgaris ( Labiatae family) or self-heal is traditionally used for different ailments such as eye pain and inflammation, headache, dizziness, sore throat and wound healing. Total phenolic and total flavonoid contents of extracts (methanol, ethanol and aqueous) were determined by a spectrophotometer. The antimicrobial activity was evaluated by micro broth dilution assay. The total phenolic content of P. vulgaris extracts were higher in aqueous extract (156.5 mg GAC/g) followed by ethanol extract and methanol extract. The TFC content of P. vulgaris methanol extract (82.8 mg QE/g) was higher than ethanol extract (22.7 mg QE/g) and aqueous extract (16.2 mg QE/g). The antimicrobial activity of methanol or ethanol extracts was higher than aqueous extract from P. vulgaris. The sensitivity of microorganisms to different extracts is related to type of pathogens. There is no positive relation between total phenolic content and its antimicrobial activity. Prunella vulgaris ethanolic extract as a source of phenolic and flavonoid contents can be used as an antimicrobial agent
3
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Chemical composition, antioxidant and antimicrobial activity of Nepeta persica Boiss. essential oil

100%
Mahboubi M., Kazempour N., Ghazian F., Taghizadeh M.
Herba Polonica
|
2011
|
tom 57
|
nr 1
The essential oil from aerial parts of Nepeta persica Boiss was obtained by steam distillation and was analyzed by GC and GC/MS. Fourteen components were identified as accounting for 97.3% of total oil composition. The 4aα,7α,7aα-nepetalactone (80%) and Spiro[5.6]dodecane (14.2%) were the main components of essential oil. Antimicrobial activity of essential oil against different kinds of microorganisms was determined by micro-broth dilution assay. The minimal inhibitory concentration and minimal lethal concentration values of oil were in the ranges from 1-8 to 1-16 μl/ml, respectively. Most of all, the oil was sensitive to Candida albicans, Staphylococcus aureus and Klebsiella pneumoniae. Antioxidant activity was evaluated by 2, 2’-diphenyl-1-picrylhydrazyl (DPPH) free radical scavenging. The IC50 values of N. persica essential oil and BHT were nearly 0.03%, 2.98%, respectively and in β-carotene/linoleic acid system, the essential oil did not show effective antioxidant activity.
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