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Aspergillus parasiticus is one of the most common fungi which contaminates peanuts by destroying peanut shells before they are harvested and the fungus produces aflatoxins. The aim of this study was to evaluate the antifungal activities of seventeen essential oils on the growth of the aflatoxigenic form of A. parasiticus in contaminated peanuts from commercial outlets in Georgia. The agar dilution method was used to test the antifungal activity of essential oils against this form of A. parasiticus at various concentrations: 500; 1,000; 1,500; 2,000; 2,500 ppm. Among the seventeen essential oils tested, the antifungal effect of cinnamon, lemongrass, clove and thyme resulted in complete inhibition of mycelial growth. Cinnamon oil inhibited mycelial growth at ≥ 1,000 ppm, lemongrass and clove oils at ≥ 1,500 ppm and thyme at 2,500 ppm. However, cedar wood, citronella, cumin and peppermint oils showed partial inhibition of mycelial growth. Eucalyptus oil, on the other hand, had less antifungal properties against growth of A. parasiticus, irrespective of its concentration. Our results indicate that the aflatoxigenic form of A. parasiticus is sensitive to selected essential oils, especially cinnamon. These findings clearly indicate that essential oils may find a practical application in controlling the growth of A. parasiticus in stored peanuts.
Sorghum is an important cereal produced as staple diet in Karnataka state of India and is prone to fungal infection during pre- and postharvest period. This paper reports the frequency and relative percentage of fungi associated with sorghum grain harvested in Karnataka State in 2004 and 2005. A total of 44 sorghum samples were analyzed for postharvest fungi by direct plating method on PDA and MGA 2.5agar medium. The genera Fusarium and Aspergillus were the mostfrequently isolated on sorghum grain. The other genera included Alternaria, Phoma, Curvularia, Penicillium andDrechslera. The data revealed a high frequency of Fusarium species(93.2%) and Aspergillus species (88.6%) with a relative percentage of23.3 and 19.6% among the 19 fungal genera recorded, respectively. The predominant fungal species recorded at high frequency were F.verticillioides (88.6%), A. flavus (72.7%), F. anthophilum (65.0%),A. niger (59.1%). These data indicate possible health hazards for humans and animals upon consumption of such contaminated food grain by toxigenic moulds.
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