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Cytological characteristics of F2 hybrids between Triticum aestivum L. and T. durum Desf. with reference to wheat breeding

100%

Wang H. Y., Liu D.-C., Yan Z.-H., Wei Y.-M., Zheng Y.-L.

Journal of Applied Genetics

2005

tom 46

nr 4

365-369

Cytological and agronomic characteristics of a F₂ population from Triticum aestivum L. × T. durum Desf. hybrids were analyzed plant by plant. Means of morphologic traits in the F₂ population were similar to those of the low-value parent. On average, F₂ hybrids had 36.54 chromosomes per plant, indicating that each gamete lost 2.73 chromosomes at meiosis of the F₁ generation. More than half of plants had 36-39 chromosomes, so male gametes with 19-21 chromosomes seemed to be superior to the others. The distribution frequency of chromosomes in this study differed from that in a previous report, where a different tetraploid wheat was used. This shows that a different breeding strategy may need to be taken when exploiting a different tetraploid wheat. According to our results, some plants with 42 chromosomes, having all the wheat A, B and D chromosomes, would appear in the F₃ population, which provides a chance to obtain stable bread wheat lines from the self-pollinated progenies. Alternatively, the desirable individuals of the F₂ population were backcrossed to bread wheat, which is very useful and efficient for the improvement of bread wheat by exploiting desirable genes in durum wheat.

Isolation of low-molecular-weight glutenin subunit genes from wild emmer wheat [Triticum dicoccoides]

86%

Yue Y. W., Long H., Liu Q., Wei Y.-M., Yan Z.-H., Zheng Y.-L.

Journal of Applied Genetics

2005

tom 46

nr 4

349-355

Three low-molecular-weight glutenin subunit (LMW-GS) genes, designated LMW-Tdl, LMW-Td2 and LMW-Td3, were isolated from wild emmer wheat (Triticum dicoccoides), which is the tetraploid progenitor of common wheat (T. aestivum). The complete nucleotide sequence lengths of LMW-Td1, LMW-Td2 and LMW-Td3 are 858, 900 and 1062 bp, respectively. LMW-Td1 and LMW-Td3 can encode proteins with 284 and 352 amino acid residues, respectively, whereas LMW-Td2 is a putative pseudogene due to the presence of 3 inframe stop codons in its C-terminal domain. The deduced protein sequences of the 3 genes share the same typical polypeptide structures with known LMW-GS genes containing 8 cysteines in the mature protein domains. LMW-Td1 was clearly distinguished from all known LMW-GS genes, and considered as a novel LMW-GS gene. Two hydrophobic motifs (i.e. PIIIL and PVIIL) were observed in the repetitive domain of LMW-Td3. Sequence comparison indicates that sequences of the 3 LMW-GS genes from this study are strongly similar to known LMW-GS genes. Our phylogenetic analysis suggests that LMW-Td1 and LMW-Td2 are homologous with genes on chromosome 1A, and LMW-Tdl is closely related to genes on chromosome 1B.

Ograniczanie wyników

2 Journal of Applied Genetics

2 Wei Y.-M.

2 Yan Z.-H.

2 Zheng Y.-L.

1 Liu D.-C.

1 Liu Q.

1 Long H.

1 Wang H. Y.

1 Yue Y. W.

2 2005

Wyniki wyszukiwania

Cytological characteristics of F2 hybrids between Triticum aestivum L. and T. durum Desf. with reference to wheat breeding

Isolation of low-molecular-weight glutenin subunit genes from wild emmer wheat [Triticum dicoccoides]