The aim of the present study was to investigate the physicochemical, the antioxidant and the antibacterial properties of three Algerian honeys (Eucalyptus, Wild carrot and Multifloral). Several physicochemical parameters including moisture content, pH, electrical conductivity (EC), Hydroxymethylfurfural (HMF), invertase number and diastase number were measured. Total phenolic contents, reducing power and ABTS scavenging activity were determined. The agar incorporation method was used to determine the antibacterial activity of honeys against three strains of E. coli isolated from diarrhea in young calves. The results showed that moisture contents vary from 15.4% to 18.0%, pH values ranged between 4.19 and 4.34, HMF contents ranged between 11.2 and > 100 mg kg-1, invertase number showed values of 3.2 and 20.7, electrical conductivity ranged between 0.38 and 1.1 mS cm-1 and diastase number was detected only in Wild carrot honey (11.3). This honey showed the highest level of polyphenols (850.48 ±167.29 mg gallic acid/kg) and the highest reducing power (0.771 ± 0.141), while Eucalyptushoney showed the best ABTS scavenging activity (1.7637 ± 0.8596 mmol Eq Trolox/L-1). A strong correlation was found between total phenolic content and reducing power (r value was 0.875 and P < 0.001). All honeys were effective against all the tested strains with Minimum Inhibitory Concentrations (MIC) of 7% and 8%. Eucalyptus honey was bactericide against all the tested strains. This study demonstrated remarkable variation in antioxidant properties of honey depending on its botanic or geographic origin. It also revealed that Algerian honeys exhibit a strong antibacterial activity
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