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Melanophilin (MLPH) gene has been characterized as one of the candidate genes for coat colour dilution in human, mice and dog, but little is known about it in goat. Part of the 5’UTR (650bp,JQ743911) was sequenced leading to identify four polymorphic loci (g.G426A, g.A570G, g.G592T and g.612-614insCTC). The genetic diversity was analysed in 120 individuals of four breeds of goat. The possible transcription factors in the four loci were mined by online tools. The results show that the four loci were in Hardy-Weinberg equilibrium (P>0.05) and there was a significant difference in gene sequence between Jining Grey (JN) and Leizhou Black (LZ) breeds (P < 0.05). JN has the highest allele frequency of A, A, G and insertion CTC at the four loci; and LZ has the highest allele frequency of G, G, T and deletion CTC. JN has the lowest effective allele number and the lowest value of Shannon I at the four polymorphic loci. LZ has the highest effective allele number and the highest Shannon I at the four polymorphic loci. Moreover, JN has the highest Fst and Gst (0.08 and 0.12, respectively) and the lowest Nm (2.80) compared to LZ. In silico analysis showed that the possible transcription factors having a potential binding site in the sequence consisting of A, A and G and insertion CTC in the four polymorphic loci of 5’UTR of MLPH gene were BRN2, CCAT,P, STAT and USF. The sequence consisting of G, G, T and deletion CTC in the 5’UTR region of MLPH formed the possible binding sites of MyoD, c-Myb, v-Myb, AP-1, USF and NKX25. It could be concluded that JN has different gene sequence in the four mutation sites of 5’UTR of MLPH compared to LZ and the mutation caused the variation of possible transcription factors that may play a role in the MLPH gene expression regulation; so JN has dilute, ashen and grey coat colours and LZ is of solid black colour.
Sugar content largely determines watermelon fruit quality. We compared changes in sugar accumulation and activities of carbohydrate enzymes in the flesh (central portion) and mesocarp of elite sweet watermelon line 97103 (Citrullus lanatus subsp. vulgaris) and exotic nonsweet line PI296341-FR (C. lanatus subsp. lanatus) to elucidate the physiological and biochemical mechanisms of sugar accumulation in watermelon fruit. The major translocated sugars, raffinose and stachyose, were more unloaded into sweet watermelon fruit than non-sweet fruit. During the fruit development, acid a-galactosidase activity was much higher in flesh of 97103 than in mesocarp of 97103, in flesh and mesocarp of PI296341-FR fruit. Insoluble acid invertase activity was higher in 97103 flesh than in 97103 mesocarp, PI296341-FR flesh or mesocarp from 18 days after pollination (DAP) to 34 DAP. Changes in soluble acid invertase activity in 97103 flesh were similar to those in PI296341-FR flesh and mesocarp from 18 DAP to full ripening. Sucrose synthase and sucrose phosphate synthase activities in 97103 flesh were significantly higher than those in 97103 mesocarp and PI296341- FR fruits from 18 to 34 DAP. Only insoluble acid invertase and sucrose phosphate synthase activities were significantly positively correlated with sucrose content in 97103 flesh. Therefore, phloem loading, distribution and metabolism of major translocated sugars, which are controlled by key sugar metabolism enzymes, determine fruit sugar accumulation in sweet and non-sweet watermelon and reflect the distribution diversity of translocated sugars between subspecies.
Aneuploidy often presents large variations in morphology, physiology, biochemistry, and genetics owing to karyotypic imbalance. This study aimed to evaluate the efficacy of aneuploid breeding in Echinacea purpurea L, an important medicinal plant. Reciprocal crosses between diploid and triploid plants were performed to generate aneuploid plants. Cross with triploid as female parent resulted in increased production of aneuploid individuals (19 of 23; 82.61%), while using diploid as female parent yielded much higher percentage of diploid progenies (130 of 133; 97.74%). Each aneuploid had particular karyotypic characteristics compared to the parents. The proportions of median, submedian, and subterminal centromere location chromosomes in gross chromosomes among aneuploids and two parents showed large variations. Although aneuploids had relatively lower adventitious bud regeneration rates than their parents, almost half of them looked morphologically normal, with high survival rates when transplanted to ex vitro conditions. Among the bioactive compounds assessed, cichoric acid and chlorogenic acid contents were extremely encouraging. Most aneuploids had higher cichoric acid and chlorogenic acid contents than their parents. For example, A2 had the highest cichoric acid content of 21.98 mg/g dry weight, more than twice the values of diploid and triploid. Meanwhile, A21 had the highest chlorogenic acid content of 1.84 mg/g, approximately five times more than the parental values. Eleven superior aneuploid lines were successfully screened as breeding candidates. The present findings indicated E. purpurea is highly tolerant of karyotypic imbalance and aneuploid plants could serve as prospective breeding resources in E. purpurea.
This study established a K drought monitoring model to determinate the start, duration, and strength of drought. Based on 586 meteorological stations’ conventional observation data in China from 1961 to 2015, we calculated the elements and parameters needed to structure the model. Because soil has a memory of its antecedent moisture change, the drought intensity of a day is determined by the moisture content of the day and earlier days. Furthermore, the change of moisture content depends not only on precipitation but also on the temperature associated with evaporation. In order to consider the impact of previous precipitation and evaporation on the present drought, we made a cumulative treatment of previous precipitation and reference evapotranspiration. However, the influence degree of the antecedent precipitation and reference evapotranspiration on soil moisture is weakened closer to the present day. This means that the contribution of the precipitation and reference evapotranspiration in earlier days to present soil moisture decays over time. After comparison between the exponential decay form and the linear decay form of the previous precipitation and reference evapotranspiration, the scheme of linear decay form is selected. Then, a K drought monitoring model was established to consider the antecedent precipitation and reference evapotranspiration accumulation and their decay influence. The proposed K drought monitoring model can be used to evaluate the timing of drought onset, evolution process, and severity.
As a member of tyrosinase-related family, tyrosinase-related protein-1 (TYRP1) has been reported to play an important role in melanin biosynthetic pathway in some species, but little is known about its role in goat. In this study, almost complete goat TYRP1 gene (17554 bp, HMO70243) encompassing complete CDS was identified, the coding region amounting to 1614 bp, distributed in 7 exons (2-8), and intron 5 was found to possess GC-AG as its splice sites. Dozens of SNPs as well as simple sequence repeat (SSR) motifs were detected in goat TYRP1 gene, in which 4 SSR motifs in intron 5 are combined in a tandem. Correlation analysis showed the allele A to be advantageous compared to C at site g.1263A>C and allele C compared to T at g.1428C>T, implyimg that haplotype AC is in favour of eumelanin biosynthesis, and haplotype CC is in favour of pheomelanin formation.Marked deficiency of heterozygotes occurred in Nanjiang Yellow Goat Black strain and Nanjiang Yellow Goat Fast Grow strain, indicating the high inbreeding in both strains.
This study investigated the role of autophagy in the survival of the invasive bacterium Brucella melitensis strain 16M in murine macrophages. Here, Brucella melitensis 16M was found to trigger autophagosome formation, enhance autophagy flux and increase the expression level of the autophagy marker protein LC3-II. When autophagy was pharmacologically inhibited by 3-methyladenine (3-MA), Brucella replication efficiency was significantly decreased (p < 0.05). These results suggest that autophagy favors Brucella melitensis 16M survival in murine macrophages.
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