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NESTED PCR w diagnostyce przypadkow pneumocystozowego zapalenia pluc [PCP] u pacjentow z Aids

100%

Golab E., Sobolewska A., Bitkowska E., Bednarska A., Berak H., Dzbenski T. H., Horban A.

Wiadomości Parazytologiczne

2001

tom 47

nr 3

521-525

The studies were undertaken to evaluate the usefulness of a nested PCR assay in the diagnosis of Pneumocystis carinii pneumonia (PCP) in AIDS patients. To achieve the end, 51 excretions samples from the respiratory traci were collected from HIV-infected patients with respiratory symptoms, and examined for the presence of specific DNA. A portion of the mitochondrial large-subunit rRNA gene of Pneumocystis carinii was amplified with outer primers pair pAZ 102E, pAZ 102H and internal primers pAZ 102X, pAZ 102Y. Positive nested PCR results were obtained with 36 out of 51 examined samples. Some 52.8% of the positive results were obtained with samples collected from patients without clinical diagnosis of PCP. It was concluded, that the nested PCR method, being too sensitive, is not suitable for the routine diagnosis of PCP in AIDS patients.

Detection of serum Pneumocystis carinii DNA in the course of P.carinii pneumonia in experimental animals and HIV-infected patients

100%

Golab E., Sobolewska A., Bitkowska E., Bednarska A., Berak H., Dzbenski T. H.

Acta Parasitologica

2002

tom 47

nr 3

A nested PCR which amplified a part of the mitochondrial gene encoding the large subunit of rRNA of Pneumocystis carinii was used to detect P. carinii DNA in sera obtained from HIV-infected patients and immunosuppressed rats. P. carinii DNA was found in sera from nearly 52% of rats between 6th and 9th week of immunosuppressive treatment and in 12 serum samples out of 51 collected from HIV-infected patients, including 4 from clinically suspected and laboratory confirmed cases of PCP. The results of the studies indicated that DNA of P. carinii is most likely to appear in the blood in the advanced infection, however, the test for serum DNA may be of little value for the diagnosis of PCP since negative results do not exclude active infections and positive ones may be encountered in asymptomatic carriers.

Ograniczanie wyników

1 Acta Parasitologica

1 Wiadomości Parazytologiczne

2 Bednarska A.

2 Berak H.

2 Bitkowska E.

2 Dzbenski T. H.

2 Golab E.

2 Sobolewska A.

1 Horban A.

1 2002

1 2001

Wyniki wyszukiwania

NESTED PCR w diagnostyce przypadkow pneumocystozowego zapalenia pluc [PCP] u pacjentow z Aids

Detection of serum Pneumocystis carinii DNA in the course of P.carinii pneumonia in experimental animals and HIV-infected patients