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Expression of Bombyx mori nucleopolyhedrovirus ORF76 in permissive and non-permissive cell lines by a novel Bac-to-Bac-BmNPV baculovirus expression system

100%

Su W. J., Wu Y., Wu H.-L., Zhu S.-Y., Wang W.-B.

Polish Journal of Microbiology

2008

tom 57

nr 4

Open reading frame 76 of Bombyx mori nucleopolyhedrovirus (BmNPV), designated as Bm76, is a gene whose function is completely unknown. With EGFP fused to the 3' terminal of Bm76 as the reporter gene and BmNPV bacmid as the expression vector, a recombinant bacmid was successfully constructed expressing Bm76-EGFP fusion protein under the control of polyhedrin promoter in Bombyx mori cells (Bm cells), BmNPV's permissive cell line, laying the foundation for rescue experiment of Bm76 deletion mutant. Moreover, the supernatant from Bm cells transfected with the recombinant bacmid was used to infect Trichoplusia Ni cells (Tn cells), BmNPV's non-permissive cell line. Unexpectedly, the expression of Bm76-EGFP fusion protein in some Tn cells was detected, implying that viral DNA was replicated in these cells. The causes are being studied for the inability of BmNPV to produce enough viable budded viruses in Tn cells despite of viral DNA replication.

A new report of a mosaic dwarf viroid-like disease on mulberry trees in China

72%

Wang W. B., Fei J.-M., Wu Y., Bai X.-C., Yu F., Shi G.-F., Li Y.-F., Kuai Y.-Z.

Polish Journal of Microbiology

2010

tom 59

nr 1

33-36

A viroid-like disease causing mosaic leaves and dwarfism was found on mulberry plants in Zhejiang, China. Grafting of stems from infected plants onto healthy plants resulted in the same symptoms on the healthy plants. A circular small RNA (Mmd-v RNA1) was isolated from the infected plant leaves and caused identical symptoms after more than two years. Nucleotide sequencing indicated that the Mmd-v RNA1 contains 356 nt (GenBank accession no. NC_011362) without viroid characteristic regions, only sharing 30 nt sequence identity with that of the Cherry small circular viroid-like RNA 1 (isolate cscRNAl.150, GenBank accession no. DQ357628). This description is the first of viroid-like RNA infection of mulberry trees.

Ograniczanie wyników

2 Polish Journal of Microbiology

2 Wu Y.

1 Bai X.-C.

1 Fei J.-M.

1 Kuai Y.-Z.

1 Li Y.-F.

1 Shi G.-F.

1 Su W. J.

1 Wang W. B.

1 Wang W.-B.

1 Wu H.-L.

1 Yu F.

1 Zhu S.-Y.

1 2010

1 2008

Wyniki wyszukiwania

Expression of Bombyx mori nucleopolyhedrovirus ORF76 in permissive and non-permissive cell lines by a novel Bac-to-Bac-BmNPV baculovirus expression system

A new report of a mosaic dwarf viroid-like disease on mulberry trees in China