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1

Studies on seasonal abundance of diamondback moth Plutella xylostella (Lepidoptera: Yponomeutidae) on cauliflower crop

100%
Ahmad T., Ansari M. S.
Journal of Plant Protection Research
|
2010
|
tom 50
|
nr 3
The studies on seasonal abundance of diamondback moth Plutella xylostella were conducted in two years 2004-2005 and 2005-2006. Survey data of three localities of Aligarhdistrict showed that initial infestation by P. xylostella occurred when the farmers started transplantation of cauliflower seedlings, the density of P.xylostella ranged between 0.90 to 2.38 and 0.27 to 5.84 larvae and pupae/plant in I week of July, 2004 and 2005, respectively, and the rate of parasitization was quite low. Temperature and humidity recorded maximum and minimum i.e. 24.15° to 32.91°C and 68.60 to 91.30 percent, respectively.Population build up is usually observed in II to IV week of September. Cotesia plutellae was found to be a dominant larval parasitoid while, Oomyzus sokolowskii parasitized relatively few pupae of P. xylostella.34.77°C significantly (p < 0.01) enhanced the population of DBM also on 8thSeptember, 8th October, 2004 and 26th January, 2005. Rainfall negatively affected the DBM population in 2004-2005 and 2005-2006.
2

Comparison of egg yolks from three avian species in extender for cryopreservation of Sahiwal bull epididymal spermatozoa

81%
Akhter S., Rakha B. A., Andrabi S. M. H., Ansari M. S.
Animal Science Papers and Reports
|
2011
|
tom 29
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nr 2
The present study was designed to compare egg yolks from three avian species in extender for cryopreservation of Sahiwal bull epididymal spermatozoa. The study was conducted on cauda epididymal spermatozoa from ten slaughtered Sahiwal bulls. Semen retrieved from epididymal cauda of each testes pair was pooled and diluted in tris-citric acid-glycerol extender containing either pigeon, guinea fowl or chicken egg yolk semi colon leading to a final spermatozoal concentration of 30×106 ml-1. Diluted semen was transferred into 0.5 ml straws, cooled to 4°C and equilibrated for 4 h. The straws were kept on liquid nitrogen vapours for 10 min and then plunged into liquid nitrogen for storage. Motility, plasma membrane integrity and spermatozoa morphology (acrosome ridge;head, mid piece and tail) of each diluted semen sample were assessed at 0, 2 and 4 h post-thawing.All indicators applied and acrosomal integrity were the highest (P<0.05) after freezing in extender containing pigeon egg yolk compared to guinea fowl and chicken egg yolk 0, 2 and at 4 h postthaw.Moreover,spermatozoa tail abnormality percentages were also significantly lower (P<0.05) in extender containing pigeon egg yolk compared to guinea fowl and chicken egg yolk at 0, 2 and 4 post-thaw h. In conclusion, pigeon egg yolk used in semen extender improved the post-thaw quality of Sahiwal bull epididymal spermatozoa.
3

Cryopreservation of bull semen in OptiXcell and conventional extenders: Comparison of semen quality and fertility

81%
Ansari M. S., Rakha B. A., Akhter S.
Animal Science Papers and Reports
|
2017
|
tom 35
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nr 3
Commercially available OptiXcell® extender was compared with conventional extenders for freezability and in vivo fertility of bull semen. Semen was collected from three Friesian bulls for five weeks (replicate) and qualifying ejaculates (motility >60%, concentration >0.5 billion/mL, volume >lmL) were diluted (37°C; 50 x 10⁶ spermatozoa/ml) with OptiXcell®, tris-citric egg yolk and egg yolk-citrate extenders. Diluted semen was cooled to 4°C in 2 hours, equilibrated for 4 hours and filled in 0.5 ml straws. The straws were kept over liquid nitrogen vapours for 10 minutes and plunged into liquid nitrogen. Percentages of post thaw sperm motility and plasma membrane integrity were recorded higher (P<0.05) in OptiXcell® compared to tris-citric egg yolk followed by egg yolk-citrate extender. Sperm viability (%) were recorded higher (P<0.05) in OptiXcell® compared to tris-citric egg yolk and egg yolk citrate extender. Percentages of normal apical ridge and DNA integrity were higher (P<0.05) in OptiXcell® and tris-citric egg yolk extender compared to egg yolk-citrate extender. Higher (P<0.05) fertility rate was recorded with semen frozen in OptiXcell® compared to triscitric egg yolk and egg yolk-citrate extender. In conclusion, OptiXcell® is superior to conventional extenders for spermatozoa) quality of frozen-thawed bull semen and produced higher fertility rates under field conditions.
4

Effect of L-cysteine in extender on post-thaw quality of Sahiwal bull semen

81%
Ansari M. S., Rakha B. A., Akhter S.
Animal Science Papers and Reports
|
2011
|
tom 29
|
nr 3
The study was conducted to evaluate the effect of addition of L-cysteine to tris-citric acid (TCA) extender on the post-thaw quality of Sahiwal bull semen. For this purpose, two consecutive ejaculates were collected from three Sahiwal bulls using artificial vagina at weekly intervals for a period of three weeks (three replicates). Qualifying semen ejaculates were diluted (50×106 motile spermatozoa ml-1) in TCA extender having L-cysteine either 0.0 (control) or 0.5, 1.0 or 2.0 mM. Diluted semen was cooled to 4°C for 2 h, equilibrated for 4 h at 4°C, filled in straws at 4°C, kept in liquid nitrogen vapours for 10 min and then stored in the liquid nitrogen. Thawing was performed after 24 h of storage, at 37°C for 30 s. and the sperm motility, viability, plasma membrane and acrosomal integrity were assessed. Higher (P<0.05) sperm motility, viability, plasma membrane and acrosomal integrity were observed using extenders containing 1.0 or 2.0 mM compared to those containing 0.5 or 0.0 mM L-cysteine. It is concluded that addition of L-cysteine (to reach 1.0-2.0 mM) in TCA extender improves the post-thaw quality of Sahiwal bull semen.
5

Effect of straw size and thawing time on quality of cryopreserved buffalo (Bubalus bubalis) semen

81%
Ansari M. S., Rakha B. A., Andrabi S. M. H., Akhter S.
Reproductive Biology
|
2011
|
tom 11
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nr 1
6

Usefulness of powdered and fresh egg yolk for cryopreservation of Zebu bull spermatozoa

81%
Akhter S., Ansari M. S., Rakha B. A., Andrabi S. M. H.
Reproductive Biology
|
2010
|
tom 10
|
nr 3
7
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Biological parameters of onion thrips, Thrips tabaci Lindeman on onion cultivars

81%
Moraiet M. A., Ansari M. S., Basri R.
Journal of Plant Protection Research
|
2017
|
tom 57
|
nr 2
Biological parameters of the onion thrips, Thrips tabaci Lindeman were studied on the following onion (Allium cepa L.) cultivars: Nasik Red Plus N-53, Onion Dr-301 (Krishna), Onion White, and Nasik Red, at 25±1oC and 65±5% RH. Signifi cant (p < 0.05) diff erences were found in the life stages and fertility life tables on diff erent cultivars except in the pupal stages. More information about the biological parameters of T. tabaci on onion cultivars can help in designing Integrated Pest Management programs for onion thrips.
8

Evaluation of Tris–citric acid, skim milk and sodium citrate extenders for liquid storage of Punjab Urial (Ovis vignei punjabiensis) spermatozoa

61%
Rakha B. A., Hussain I., Akhter S., Ullah N., Andrabi S. M. H., Ansari M. S.
Reproductive Biology
|
2013
|
tom 13
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nr 3
9

Glutathione-supplemented tris-citric acid extender improves the post-thaw quality and in vivo fertility of buffalo (Bubalus bubalis) bull spermatozoa

61%
Ansari M. S., Rakha B. A., Anrabi S. M. H., Ullah N., Iqbal R., Holt W. V., Akhter S.
Reproductive Biology
|
2012
|
tom 12
|
nr 3
10

Effect of exogenous glutathione in extender on the freezability of Nili-Ravi buffalo (Bubalus bubalis) bull spermatozoa

61%
Ansari M. S., Rakha B. A., Ullah N., Andrabi S. M. H., Iqbal S., Khalid M., Akhter S.
Animal Science Papers and Reports
|
2010
|
tom 28
|
nr 3
The study was designed to investigate the effect of exogenous glutathione supplementation (0.0,0.5, 1.0, 2.0 and 3.0 mM) in tris-citric acid extender on post-thaw sperm motility, viability, plasma membrane and acrosomal integrity of buffalo (Bubalus bubalis) spermatozoa at 0, 3 and 6 hours after thawing. Glutathione supplementation of the extender upto 2.0 mM concentration increased (P≤0.05) sperm motility, viability, plasma membrane integrity and acrosomal integrity at 0, 3 and 6 hours after thawing in a dose-dependent manner compared to the control. However, glutathione supplementation at higher concentration (3.0 mM) was not beneficial for any of semen quality parameters. It is concluded that glutathione supplementation (up to 2.0 mM) of the extender improves the post-thaw quality of buffalo bull spermatozoa.
11

Efficiency of sucrose density gradient method for sex separation of buffalo spermatozoa as validated by SYBR Green Real Time PCR

51%
ul-Husna A., Azam A., Awan M. A., Khalid M., Sami A., Shahzad Q., Ansari M. S., Rakha B. A., Naqvi S. M. S., Akhter S.
Animal Science Papers and Reports
|
2020
|
tom 38
|
nr 1
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