The study aimed to examine resistance of 14 cucumber double haploid (DH) lines obtained from Izyd F₁ and Frykas F₁ varieties to angular leaf spot. The DH lines are valuable, homozygous breeding material. The DH lines were tested in laboratory conditions with the use of one, highly virulent Pseudomonas syringae pv. lachrymans strain. The inoculum concentration used was equal to 10⁷ CFU. Plants were scored for resistance in comparison to resistant (GY 14 line) and susceptible (B line) standards, as well as in comparison to Izyd F₁ and Frykas F₁ varieties. Seven days after inoculation the plants were scored by nine degree rating scale, where 1 means total susceptibility and 9 means the total resistance. Data were statistically processed using one-way analysis of variance. 70% of the tested DH lines showed higher resistance level to angular leaf spot than the resistant standard - GY 14 line, and were scored in rating scale above 6. These lines did not show any chlorotic lesions, whereas the necrotic lesions were found on 10-20% of leaf area. The most valuable were I 502, I 504, I 506/2, I 18/1, and I 321 lines obtained from Izyd F₁ variety, which were scored at 7-8 in rating scale; only tiny necrotic lesions without halo were visible on the leaf surface of these lines. The results showed the higher resistance to angular leaf spot in DH lines as compared with Izyd F₁ and Frykas F₁ varieties.