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Kinobeon A, purified from cultured safflower cells, is a novel and potent singlet oxygen quencher

100%

Kambayashi Y., Takekoshi S., Nakano M., Shibamori M., Hitomi Y., Ogino K.

Acta Biochimica Polonica

2005

tom 52

nr 4

We recently reported that kinobeon A, produced from safflower cells, suppressed the free radical-induced damage of cell and microsomal membranes. In the present study, we investigated whether kinobeon A quenches singlet oxygen, another important active oxygen species. Kinobeon A inhibited the singlet oxygen-induced oxidation of squalene. The second-order rate constant between singlet oxygen and kinobeon A was 1.15 × 1010 M-1s-1 in methanol containing 10% dimethyl sulfoxide at 37°C. Those of α-tocopherol and β-carotene, which are known potent singlet oxygen quenchers, were 4.45 × 108 M-1s-1 and 1.26 × 1010 M-1s-1, respectively. When kinobeon A was incubated with a thermolytic singlet oxygen generator, its concentration decreased. However, this change was extremely small compared to the amount of singlet oxygen formed and the inhibitory effect of kinobeon A on squalene oxidation by singlet oxygen. In conclusion, kinobeon A was a strong singlet oxygen quencher. It reacted chemically with singlet oxygen, but it was physical quenching that was mainly responsible for the elimination of singlet oxygen by kinobeon A. Kinobeon A is expected to have a preventive effect on singlet oxygen-related diseases of the skin or eyes.

Contribution of the calcineurin signaling pathway to overload-induced skeletal muscle fiber-type transition

100%

Miyazaki M., Hitomi Y., Kizaki T., Ohno H., Haga S., Takemasa T.

Journal of Physiology and Pharmacology

2004

tom 55

nr 4

Skeletal muscle is highly adaptable, being capable of undergoing changes in its structural and functional properties in response to physiological stimuli. The fast-to-slow muscle fiber-type transition is evoked by increased motor nerve activity. Recently, the calcineurin (CaN) signaling pathway has been implicated in the transcriptional regulation of slow muscle fiber genes. Here we investigated the effect of treatment with a CaN-specific inhibitor, FK506, on skeletal muscle fiber-type transition in functionally overloaded muscles. The overloaded plantaris muscle showed fast-to-slow muscle fiber type transition, i.e., a decrease in myosin heavy chain (MHC) IIb, an increase in MHCIIa+d/x, and new expression of MHCI. In the FK506-administered group, however, overload-induced muscle fiber-type transition was completely prevented. We have demonstrated, therefore, that the CaN signaling pathway is required for fast-to-slow skeletal muscle fiber-type transition. Furthermore, we also confirmed that the protein expression levels of downstream effectors of CaN signaling exhibit a transient increase in the early phase of the overloaded condition.

pH Profile of cytochrome c-catalyzed tyrosine nitration

84%

Kambayashi Y., Hitomi Y., Kodama N., Kubo M., Okuda J., Takemoto K., Shibamori M., Takigawa T., Ogino K.

Acta Biochimica Polonica

2006

tom 53

nr 3

Ograniczanie wyników

2 Acta Biochimica Polonica

1 Journal of Physiology and Pharmacology

3 Hitomi Y.

2 Kambayashi Y.

2 Ogino K.

2 Shibamori M.

1 Haga S.

1 Kizaki T.

1 Kodama N.

1 Kubo M.

1 Miyazaki M.

1 Nakano M.

1 Ohno H.

1 Okuda J.

1 Takekoshi S.

1 Takemasa T.

1 Takemoto K.

1 Takigawa T.

1 2006

1 2005

1 2004

Wyniki wyszukiwania

Kinobeon A, purified from cultured safflower cells, is a novel and potent singlet oxygen quencher

Contribution of the calcineurin signaling pathway to overload-induced skeletal muscle fiber-type transition

pH Profile of cytochrome c-catalyzed tyrosine nitration