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The material for the study consisted of F₁ hybrid populations derived from crosses among strawberry genotypes such as: ‘Calypso’, ‘Evita’, ‘Ostara’, ‘Selva’ and ‘Senga Sengana’. The crosses were conducted in a complete diallel design in accordance with Griffing’s first method. In the analysed model, 8 quantitative characters such as: time of flowering and ripening, number of inflorescences, flowers per inflorescence, crowns and fruits as well as yield and average fruit weight were assessed. The obtained results showed that for the majority of analysed traits a considerable role is played by non-additive genetic effects except for ripening time, for which additive genetic effects were essential. Heritability coefficients of broad sense were very high in almost all cases, whereas ones of narrow sense were low for most characters. Yield was positively correlated with number of fruits, inflorescences and crowns.
Honeyberry’s popularity has been increasing because of its valuable traits: nutritional value of fruits (a high amount of ascorbic acid, potassium and polyphenolic compounds), early ripening (14 days before strawberry), exceptional hardiness and easy cultivation. However, there is still little known about the pollination biology of this species, which is essential for practical breeding purposes. In this study we evaluated pollen fertility in five cultivars and three breeding clones of this species. The impact of the manner of pollination on the fruit set and forming seeds has also been studied. The highest percentage of viable pollen was found in cultivars ‘Duet’ and ‘Wojtek’ (96%), whereas the pollen of ‘Chelyabinka’ cultivar had the lowest fertility (90%). The percentage of blue honeysuckle fruits obtained from open-pollinated flowers was high (on average 94.7%) compared to isolated flowers (on average 8.6%). The effect of cross-pollination with cv. ‘Duet’ indicates that the percentage of the fruit set, fruit weight and mass of produced seeds was higher when this cultivar was applied as maternal than paternal form. The number of seeds affected significantly the weight of the fruit in the case when cv. ’Duet’ was used as a paternal (correlation coefficient r = 0.45) or maternal form (r = 0.66), while for other methods of pollination this effect was insignificant. These results may be used to the most efficient establishment of plantations as well as for the realization of the breeding program of this valuable species.
The aim of this study was to develop an inbred lines from octoploid strawberry and to determine their breeding value by estimating the effects of general combining ability (GCA) and mid-parent heterosis. All inbred lines were obtained through selfing using five cultivars. The number of inflorescences, number of flowers per inflorescence, fruit yield, fruit number per plant, average fruit weight, number and weight of leaves as well as number of runners per plant were recorded from 2014 to 2015. The highest breeding value was estimated for maternal forms of ‘Senga Sengana’ 17 for six important traits. The lowest GCA effects were exhibited by inbred lines derived from clone 1387 18. Degree of heterosis for a given trait differed greatly among hybrids. The highest heterosis in terms of a number of inflorescences, fruit yield, number of fruits per plant and leaf weight occurred in the Kent 7-14 × ‘Dukat’ progeny. The results obtained could be used to evaluate the agronomic performance and to make more efficient choices of parents in current strawberry breeding programs
The aim of the research was to assess genetic control, heritability and correlation of some quantitative characters on the basis of hierarchic crossing scheme. In the experiment two systems of classification were applied. In the first one superior classification was made according male forms (5♂ ×4♀) and in the second one female forms were superior (10♀ ×3♂). Genetic control for most analysed traits was the same regardless of classification system. Only in case of yield genetic control was different in paternal than in maternal classification. Additive genetic components were essential in determining the time of flowering, whereas non-additive ones in determining the number of stolons. The high value of broad-sense heritability for most cases suggests large genetic influence on analysed characteristics.
In the present study genetic diversity between 19 blueberry cultivars was evaluated using Randomly Amplified Polymorphic DNA (RAPD) and Inter Simple Sequence Repeat (ISSR) markers. In total, nine selected RAPD primers produced 89% polymorphic fragments, whereas eleven ISSR primers – 82%. Resolving power value of the RAPD primers ranged from 2.40 to 7.19, whilst ISSR from 1.90 to 5.78. The similarity coefficients estimated on the basis of the two types of marker systems were very similar, on average amounting to 0.58 for RAPD and 0.60 for ISSR analysis. Cluster analysis based on RAPD markers showed that the 19 accessions can be classified into 6 groups. Taking into account corresponding levels of average genetic similarity (0.59) it is possible to identify three main clusters based on ISSR analysis. The RAPD as well as ISSR markers revealed the existence of genetic differentiation between accessions, which can be exploited in hybridization programs of this species.
Celem pracy było określenie wartości produkcyjnej wyselekcjonowanych klonów hodowlanych truskawki. Analiza takich cech, jak: termin zakwitania, termin dojrzewania, obfitość kwitnienia, plon owoców, masa pojedynczego owocu wyboru ekstra, procentowy udział owoców ekstra w plonie, kształt owocu, barwa skórki, barwa miąższu, jędrność wykazała znaczne zróżnicowanie pomiędzy badanymi genotypami. Zespołem korzystnych właściwości charakteryzowały się klony hodowlane o numerach: 1596, 2395, 3995.
In principle, in vitro propagation results in uniform batches of plants, which grow, flower and fruit normally. However, phenotypic changes often observed in the field performance of tissue culture-produced strawberry plants might affect their agronomic value. So, it is of utmost importance to assess the field performance of in vitro propagated plants to verify their fidelity to conventional propagated plants. In this study, the agronomic value of the strawberry plants derived from cultivars ‘Filon’ and ‘Teresa’ via in vitro propagation and their first vegetative progeny was compared to conventional plants. During the field experiments, agronomic traits such as plant vigor, abundance of flowering and yield components were evaluated. The results showed the different field response of cultivars tested to in vitro propagation. In spite of the phenotypic changes observed in in vitro derived plants, their agronomic value was equal or superior in comparison with conventional plants. In conclusion, it should be stated that in vitro propagation method can be safely recommended for the reproduction of these strawberry cultivars.
During the years 2012 to 2014 some research connected with highbush blueberry was taken. It described phenotypic and genotypic variability of 19 cultivars of highbush blueberries grown in the Lublin region. The cultivars included in the study were: ‘Bluecrop’, ‘Bluejay’, ‘Blueray’, ‘Bonifacy’, ‘Bonus’, ‘Brigitta Blue’, ‘Chandler’, ‘Chanticleer’, ‘Croatan’, ‘Darrow’, ‘Duke’, ‘Earliblue’, ‘Herbert’, ‘Jersey’, ‘Northland’, ‘Nelson’, ‘Patriot’, ‘Toro’ and ‘Spartan’. The flowering of highbush blueberry can be observed from the end of April to the beginning of June. Meanwhile, ripening depended on cultivar, starting from June 29th and finishing by September 7th. The strongest vegetative growth was characteristic of ‘Bluecrop’ cultivar on the oldest plantation – Niemce, whereas on the Spiczyn 1 – ‘Darrow’ cultivar, and ‘Patriot’ proved to be the best yielding cultivar on the youngest plantation. In the study, berries of the ‘Chandler’ cultivar were the largest, and berries of the ‘Northland’ cultivar were considered to be the smallest. To evaluate the tested cultivars at the DNA level RAPD markers were used. The set of 9 analysing primers generated a total of 91 fragments of which 81 (89%) were polymorphic. The average genetic similarity determined on the basis of the similarity matrix of RAPD markers was 0.41. Application of the UPGMA method for grouping varieties showed the highest distinction of cultivars: ‘Croatan’, ‘Chanticleer’, ‘Herbert’ and ‘Brigitta Blue’, in relation to the others. Among the tested cultivars genetic variation was detected since genetic similarity ranged from 0.22 to 0.60. Nevertheless the same cultivars grown in different locations demonstrated genetic identity.
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Nutraceutical value of the new strawberry cultivars

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Regarding an increasing interest of consumers towards berry fruit, as they have been proved to have potential health benefits, the nutraceutical fruit value of seven strawberry cultivars: ‘Felina’, ‘Feltar’, ‘Hulta’, ‘Jota’, ‘Pastel’, ‘Plena’ and ‘Teresa’ selected at the University of Life Sciences in Lublin, Plant Genetics and Breeding Department was evaluated in this study. In fruits harvested at maturity, the level of phytochemical compounds such as sugars, vitamin C, and polyphenols including quercetin and gallic acid as well as antiradical activity was estimated. Among all genotypes tested, the cultivar ‘Jota’ showed the highest content of glucose and fructose as well a high content of sucrose, therefore the total sugar content exceeded 12 g per 100 g of fresh weight (FW). Similar parameters in terms of content of total sugars and the individual fractions have cultivar ‘Pastel’. Cultivars with low sucrose content were ‘Feltar’ and ‘Teresa’, but as regards monosaccharide’s cvs. ‘Plena’ and ‘Felina’. The content of vitamin C in fruits of the cultivars tested showed significant differences. It ranged from 64.5 mg·100 g⁻¹ FW in cv. ‘Jota’ to 104.33 mg·100 g⁻¹ FW in cv. ‘Plena’. The highest level of antiradical activity occurred in cultivars ‘Jota’, ‘Teresa’ and ‘Feltar’ (90.1%, 87.1% and 86.3% DPPH; respectively). The results obtained indicated that the differentiation of the chemical composition of strawberry fruit is clearly dependent upon the genetic diversity of cultivars tested, what we argue that all of them grown under the same climatic and soil conditions using the same agricultural technology.
Blue honeysuckle due to the high health benefits of its fruit, early ripening (before the first strawberries cultivars) and high frost resistance of both plants and flowers has gained the great popularity both in cultivation and breeding. The implementation of the breeding program of new cultivars requires the knowledge of the characteristics that determine its direction in the selected material. Therefore, in this study the evaluation of the phenological phases, yield, fruit weight, number of seeds per fruit and pollen viability in the cultivars and breeding clones of this species was made. The tested cultivars and clones had different levels of the analysed qualities except the pollen viability which was high but did not differ significantly within the genotypes. The evaluation of such characteristics as yield potential and fruit weight indicates that cultivars ‘Warszawa’, ‘Wojtek’ and T2 clone which had higher values of these characteristics as compared to other genotypes are possible to be used in breeding programme. Obtainment of the forms with early fruit ripening can be realised through the use of the selected Russian cultivars. The objective of this study was also to characterise the blue honeysuckle germplasms using RAPD markers and to assess their genetic similarity. The analysed primers produced 61 fragments out of which 57 (93.44%) were polymorphic. The genetic similarity matrix was made on the basis of RAPD markers. The mean genetic similarity was calculated at 0.56. The presented study confirms that the use of RAPD markers is a practical and effective method to evaluate the genetic similarity of blue honeysuckle genotypes and to establish genetic relationships between these genotypes.
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