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In the spring of 2012, sophora (Sophora alopecuroides L.) plants showing symptoms of leaf yellowing, little leaves and stunting were observed in Firooz-kuh (Tehran province), Sari (Mazandaran province) and Urmia (West Azerbaijan province) in Iran. Symptomatic plants from the three locations were subjected to nested polymerase chain reaction (PCR) to amplify 16SrRNA using primer pair P1/P7 followed by primer pair R16F2n/R16R2. Th e amplicons were purifi ed, sequenced and the nucleotide sequences were analyzed by virtual restriction fragment length polymorphism (RFLP). Th e phytoplasmas associated with the yellows disease were identifi ed as members of the 16SrIX group (Candidatus Phytoplasma phoenicium) and the 16SrXII group (Candidatus Phytoplasma solani). Th e two phytoplasmas were placed in 16SrIX-C and 16SrXII-A subgroups, respectively, in constructed phylogenetic trees. Th is is the fi rst report on sophora yellows associated with Candidatus Phytoplasma phoenicium.
Pathogenesis related (PR) genes of rice are among the most important defense genes in the interaction of rice with pathogens. In this study, the role of NH-1, several PR genes, phenylalanine ammonia-lyase (PAL), and lipoxygenase in the defense responses of rice against Rhizoctonia solani, the causal agent of rice sheath blight disease, was evaluated. The Tarom and Khazar cultivars (cvs), as resistant and susceptible genotypes, respectively, were used. The expression rate of defense genes in two-week-old seedlings inoculated with a virulent isolate of R. solani AG-I-1 A was investigated. The lesions in the Tarom cv were less than half the size of those on the Khazar cv. The expression scripts of the genes were calculated by quantative Real-Time PCR (RT-PCR). Results showed that the expression rate of all genes in the resistant cultivar was higher than that of the susceptible genotype, post inoculation. Analysis of data by the t-Student test also indicated significant differences in the expression level of the genes between Khazar and Tarom. The results of this study suggest that the investigated genes are involved in the resistance responses of rice against the sheath blight agent. For the first time, the induction of PR-5, PR-9, PR-10, PR-12, PR-13, and NH-1 was observed in this study in the resistant and susceptible Iranian cultivars of rice following attacks by R. solani.
Hairy root disease is caused by the infection of wounded higher plants with Agrobacterium rhizogenes. Transformation of tissues or plants with A. rhizogenes, and with rol genes, as well as hairy roots may produce alterations in the plant secondary metabolism. H₂O₂ and other ROS are involved as a signal in secondary metabolite production pathway and play a key role in plant defensive reactions. In this work, the effect of A. rhizogenes T-DNA on nicotine content, antioxidant enzymes activity, H₂O₂ production, pattern of peroxidase (POX) and superoxide dismutase (SOD) isozymes in hairy roots and regenerated plants were studied. Rise in SOD and POX activities in the transformed lines of TRa and TRb and in the resultant regenerated plants, also the decreased level of H₂O₂ in them, compared with the untransformed lines indicates that, the T-DNA genes expression of A. rhizogenes probably decreases H₂O₂ level by increasing the production of antioxidant enzymes. Decrees the level of H₂O₂ content in TRc line in spite of the similarity of antioxidant enzyme activity in comparison with normal root, indicate that A. rhizogenes activate other mechanisms except SOD and POX enzyme for reducing H₂O₂ level.
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