During 19-day study with broiler poultry, broiler chicks were fed diet containing clinoptilolite as supplement at the concentrations of 3% and 5%. At the same time, the following parameters were observed: activity of alkaline phosphatase (ALP) and body weight of broiler chicks. ALP activity in broiler chicks (groups G4, G5, and G6) fed aflatoxin B₁ was enhanced during all days of the observation in comparison with the control broiler chicks G1. Significant increase in ALP (P ≤ 0.05) was recorded from day 42 of the age onwards in group G4 of broiler chicks in comparison with the control group G1. Significant increase in ALP (P ≤ 0.01) was recorded from day 42 of the age onwards in group G5 of broiler chicks in comparison with the broiler chicks in group G2 and significant increase in ALP (P ≤ 0.01) was recorded onwards in group G6 of broiler chicks in comparison with the broiler chicks in group G3. Significant increase in ALP (P ≤ 0.05) was recorded from day 49 of the age onwards in group G5 of broiler chicks in comparison with the broiler chicks in group G2 and in group G6 of broiler chicks in comparison with the broiler chicks in group G3. The body weight of broiler chicks from group G2 (supplementation with 3% clinoptilolite) was 229.33 g higher in comparison with the control group G1. In group G3 fed feed mixture supplement with 5% concentration of clinoptilolite, the final mean body weight was only 179.33 g higher in comparison with the control group G1. The results of our study suggest that clinoptilolite as mineral non-nutritional supplement in feed mixture may protect from the effect of aflatoxin B₁ in young growing broilers. The supplementation of poultry diet with 3% clinoptilolite proved to be a suitable method to reduce the risk of the adverse effects of aflatoxin B₁ on poultry organism.
Effect of both vitamin D₃ and cadmium (Cd) on chymotrypsin activity (ACHT) of laying hen has been studied. Ten month-old laying hens were fed a compound feed of HYD-10 ad libitum. The birds were divided into 8 groups (each group consisting of 6 birds) as follows: experimental groups G1, G3, and G5 were supplemented with Cd (0.3; 0.6; and 6.0 mg.kg⁻¹ CdCl₂, respectively) groups G2, G4, and G6 were supplemented with vitamin D₃ (100 I.U./day) at the same Cd concentrations. C₁ was control group without any supplements and C₂ was control group supplemented only with vitamin D₃. The study lasted 6 months. In groups G1-G4, ACHT activities in the end of the study were rather high (2097.4; 2984; 1805.4, and 2575.9 U.g⁻¹,respectively). The ACHT activity in groups G2, G4 and G6 was by little higher than that in groups G1, G3, and G5 without vitamin D₃ supplementation. The principle of the action of vitamin D₃ via Ca ions and calmodulin is further discussed.
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