Two methods used for serotyping of Listeria monocytogenes were compared: 1) in-house method, which is used routinely in the National Reference Laboratory (NRL), Dolný Kubín, (Slovakia) and 2) the method recommended by the European Union Reference Laboratory (EURL) in Paris. Thirty strains of L. monocytogenes used for serotyping were isolated from various food categories and swabs in both the EURL (10 isolates) and in the NRL (20 isolates). They showed the following serological profiles: 18 of them (60%) belonged to serogroup 1/2; seven strains (23%) belonged to serogroup 4, and five isolates (17%) were identified as serogroup 3. The most frequent L. monocytogenes serotypes in food were l/2a and 3a, whereas in swabs serogroup 4 was predominant. No differences in the detection of somatic O antigens were noticed among L. monocytogenes isolates between two methods of serotyping. In three isolates, however, some differences were found in the presence of flagellar H antigens, which were confirmed by the in-house method but were not revealed by the EURL method. In one isolate, the presence of flagellar HD antigen was not recovered by any of two serotyping methods tested. Based on the results of this study, the in-house method is more accurate, less laborious, and more convenient for routine diagnosis than the EURL method.
JavaScript jest wyłączony w Twojej przeglądarce internetowej. Włącz go, a następnie odśwież stronę, aby móc w pełni z niej korzystać.