A total of 94 pectolytic and 60 nonpectolytic Pseudomonas isolates were obtained from 250 samples of rotted vegetable specimens representing various economically important vegetables. The isolates were identified on the basis of standard biochemical tests. Pseudomonas fluorescens biovar V and II and Pseudomonas putida were the most abundant species among pectolytic isolates and Pseudomonas fluorescens biovar I among nonpectolytic ones. Only 3 Pseudomonas viridiflava isolates were identified and all of them were obtained from potato. Isolates of pectolytic phenotype were scattered among nonpectolytic ones irrespective of their taxonomical status. Isolates identified biochemically, as Pseudomonas marginalis were also present in nonpectolytic group. PCR method is unsuitable for identification and differentiation of bacteria belonging to pectolytic fluorescens Pseudomonas group due to great diversity of species. However, the results of PCR amplification of the genes encoding pectate lyase suggest that genes responsible for production of this enzyme may also be present in isolates of nonpectolytic phenotype.
During our study we examined 19 most popular Polish potato cultivars. Susceptibility of tubers to bacterial infection was assessed by two methods: direct injection of bacterial suspension into tuber tissue and clay-inoculation method. The cultivars were divided into three groups: tolerant, relatively susceptible and susceptible. Elida, Bila and Vistula proved to be the most susceptible potato cultivars whereas Ekra and Mila the most resistant. Some of the cultivars such as Oda had resistant peel and susceptible tissue. Therefore during storage of Oda-like cultivars healthy and undamaged tubers are so important.
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