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1

Application of evaluation algorithm for port logistics park based on PCA-SVM model

100%
Hu B.
Polish Maritime Research
|
2018
|
tom 25
|
nr Special Issue S3
To predict the logistics needs of the port, an evaluation algorithm for the port logistics park based on the PCA-SVM model was proposed. First, a quantitative indicator set for port logistics demand analysis was established. Then, based on the grey correlation analysis method, the specific indicator set of port logistics demand analysis was selected. The advantages of both principal component analysis and support vector machine algorithms were combined. The PCA-SVM model was constructed as a predictive model of the port logistics demand scale. The empirical analysis was conducted. Finally, from the perspective of the structure, demand, f low pattern and scale of port logistics demand, the future logistics demand of Shenzhen port was analysed. Through sensitivity analysis, the main inf luencing factors were found out, and the future development proposals of Shenzhen port were put forward. The results showed that the port throughput of Shenzhen City in 2016 was 21,328,200 tons. Compared with the previous year, it decreased by about 1.74 %. In summary, the PCA-SVM model accurately predicts the logistics needs of the port
2

Anatomical features of the radial artery in the Xinjiang population in China and its impact on the transradial coronary intervention procedure

51%
Naman T., Li G. Q., Cheng H., Hu B.
Folia Morphologica
|
2020
|
tom 79
|
nr 2
Background: The anatomical features of the radial artery (RA) influence the transradial coronary intervention. The aim of this study is to discuss the anatomical features of the RA in the Xinjiang population and to guide interventionists in decreasing complications and improving success rates. Materials and methods: We enrolled 1731 patients in this study. All relevant basic information was recorded in detail, and the RA diameter was examined. Patients were divided into a RA variation group and a RA non-variation group; univariate and multivariate factor analyses were performed to evaluate the relevant factors for RA diameter and the predictive value of the variable factors in RA variations. Results: The mean RA diameter for all patients was 3.01 ± 0.14 mm. The multi-factorial analysis showed that height, gender, and occupation are significantly associated with RA diameter (p < 0.05). The incidence of RA variation was 4.97% (86/1731). Multi-factorial analysis showed that: gender (OR 2.72, 95% CI 1.469–5.037, p < 0.01), occupation (OR 2.228, 95% CI 1.0.000–0.012, p < 0.001) and RA inner diameter (OR 0.002, 95% CI 0.000–0.012, p < 0.001) are significantly associated with the incidence of RA variation. Conclusions: The mean RA diameter in the Xinjiang population was 3.01 ± 0.14 mm, height, gender, occupation are associated with RA diameter, gender, farmer, RA diameter are associated with RA variation. These factors can guide the interventionists to choose the appropriate equipment before the operation then can increase the efficiency of operation and prevent from the complications. (Folia Morphol 2020; 79, 2: 226–235)
3

The impact of acid deposition on China’s Three Gorges Reservoir

45%
Guo P., Wang Y., Zhang H., Wang B., Hu B.
Polish Journal of Environmental Studies
|
2015
|
tom 24
|
nr 6
4

The effect of calnexin deletion on the expression level of binding protein [BiP] under heat stress conditions in Saccharomyces cerevisiae

45%
Zhang H., Hu B., Ji Y., Kato A., Song Y.
Cellular and Molecular Biology Letters
|
2008
|
tom 13
|
nr 4
621-631
We cultured calnexin-disrupted and wild-type Saccharomyces cerevisiae strains under conditions of heat stress. The growth rate of the calnexin-disrupted yeast was almost the same as that of the wild-type yeast under those conditions. However, the induced mRNA level of the molecular chaperone PDI in the ER was clearly higher in calnexin-disrupted S. cerevisiae relative to the wild type at 37°C, despite being almost the same in the two strains under normal conditions. The western blotting analysis for PDI protein expression in the ER yielded results that show a parallel in their mRNA levels in the two strains. We suggest that PDI may interact with calnexin under heat stress conditions, and that the induction of PDI in the ER can recover part of the function of calnexin in calnexin-disrupted yeast, and result in the same growth rate as in wild-type yeast.
5

Epigenetic control of Pollen Ole e 1 allergen and extensin family gene expression in Arabidopsis thaliana

39%
Hu B., Liu B., Liu L., Liu C., Xu L., Ruan Y.
Acta Physiologiae Plantarum
|
2014
|
tom 36
|
nr 08
Pollen Ole e 1 domain-containing proteins were initially identified as major allergens. However, recent studies have suggested that these proteins also function as developmental regulators in many other plant tissues. To understand the possible roles of these proteins in plant development, we characterized 28 Pollen Ole e 1 allergen and extensin (AtPOE1) family proteins from Arabidopsis thaliana, and analyzed the transcript levels of the genes encoding these proteins in various tissues. Our analysis revealed that most of the AtPOE1 family genes showed spatial transcription patterns, suggesting that they may be involved in various events at diverse developmental stages. Using one member of the family, AtPOE1;26, as a model, we showed that it is mainly expressed in the root. In addition, the AtPOE1;26 gene locus has a significant histone H3 lysine 27 trimethylation (H3K27me3) modification, which is the typical epigenetic regulation marker of the Polycomb group (PcG) pathway. AtPOE1;26 transcription was upregulated in the PcG mutant curly leaf (clf), and the H3K27me3 level at the AtPOE1;26 locus was reduced in clf, suggesting that its expression is controlled by PcG. Besides AtPOE1;26, the gene loci of at least 13 AtPOE1 family members were modified by H3K27me3. Overall, these data indicate that AtPOE1 family members have spatial expression patterns in multiple plant tissues, and that their expression could be epigenetically controlled.
6

The higher expression and distribution of 9-cis-epoxycarotenoid dioxygenase1 (AhNCED1) from Arachis hyopgaea L. contribute to tolerance to water stress in a drought-tolerant cultivar

39%
Hu B., Hong L., Liu X., Xiao S., Lv Y., Li L.
Acta Physiologiae Plantarum
|
2013
|
tom 35
|
nr 05
The 9-cis-epoxycarotenoid dioxygenase (NCED) is thought to be the rate-limiting enzyme in the abscisic acid (ABA) biosynthetic pathway. In this study, transient expression of AhNCED1 and ABA distribution were detected in the vascular cambium of a drought-tolerant peanut cultivar (Yueyou 7) under a water stress treatment. It caused increases in ABA content in this region. The synthesis of ABA and AhNCED1 in the leaves of Yueyou 7 took place more quickly than in the control cultivar (Shanyou 523). Furthermore, AhNCED1 mRNA and proteins were induced in Yueyou 7 than in Shanyou 523, coinciding with greater ABA accumulation. During the seedling, blooming, and fruiting stages, AhNCED1 protein expression was higher in Yueyou 7 than in Shanyou 523, and it was induced more quickly when the plants were under water stress. These data suggest that the drought-tolerant cultivar can synthesize and distribute ABA more rapidly than does the control cultivar because of a high level of AhNCED1 expression, which then modulates physiological responses under water stress conditions.
7

Characteristics of N2O accumulation during the endogenous denitrification of nitrite

39%
Ding X., Zhao J., Hu B., Wang S., Li X., Lan L.
Polish Journal of Environmental Studies
|
2020
|
tom 29
|
nr 1
8

Sequence variations and expression analysis of FAD2 gene among safflower materials with different linoleic acid content in seed oil

39%
Li D., Yu J., Wang Q., Hu B., Chen C., Hou K., Wu W.
Acta Physiologiae Plantarum
|
2019
|
tom 41
|
nr 08
Safflower varieties were commonly divided into high, low and middle linoleic acid (LA) types according to their LA relative percentage contents in the seed oil. Fatty acid desaturase 2 (FAD2) plays a key role for LA content in seed. The sequence variations of FAD2 (CtFAD2-2, CtFAD2-10, CtFAD2-11) genes which could express in developmental seed of safflower were analyzed in 15 different LA-type materials. The results revealed that the CtFAD2-2 sequences were the same in all materials, and the CtFAD2-10, CtFAD2-11 sequences formed into two haplotypes independent of the LA-type of safflower seed. Yeast expression analysis revealed that two haplotypes of CtFAD2-10 had the function of oleic acid desaturase. The real-time PCR analysis of FAD2 in high and low LA-type materials at different seed developmental stages revealed that CtFAD2-2, CtFAD2-10 and CtFAD2-11 expressed mainly at 10 days after flower (DAF) for two different LA-type materials and the accumulation of few mRNA was detected in 14–22 DAF. The expression pattern of CtFAD2-1 in two different LA-type safflower materials was significantly divergent. For high-LA type, the accumulation of CtFAD2-1 mRNA was extremely low in 10 DAF, and its expression level increased sharply between 14 and 18 DAF and decreased slightly after 18 DAF. For low-LA type, the accumulation of CtFAD2-1 mRNA was extremely low during seed development stages. In conclusion, the gene structure and expression level of CtFAD2-1 may be the main factor affecting the differentiation of LA-type for safflower materials.
9

Theoretical analysis of microbial yield and pathways related to N2O production in nitrification

39%
Hu B., Tian X., Zhao J., Wu P., Yang W., Chen Y.
Polish Journal of Environmental Studies
|
2017
|
tom 26
|
nr 1
Identifying microbial yield and the pathway relating to nitrous oxide (N₂O) production in nitrification were essential prerequisites for modeling N₂O production and emissions. A thermodynamic method based on standard Gibbs free energy change of bio-chemical reaction was used to evaluate the NH₃-NO, NH₃-NO₂⁻ , NH₄⁺-NO, NH₄⁺-NO₂⁻ , NH₂OH-NO, and NH₂OH-NO₂⁻ reactions. Meanwhile, the stoichiometric equations of the relevant reactions were built up to ascertain the microbial yield of the microorganisms responsible for N₂O production. The results indicated that: 1) all the above reactions had the possibility of producing N₂O in nitrification as the standard Gibbs free energy changes of all the reactions were negative, 2) all the possible reactions can be used as the pathways to describe N₂O production in nitrification in mathematical models, and 3) theoretically, the yield coefficient of autotrophs relating to N₂O production was in the range of 0.097~0.194 g cell/ g N. Ammonia-oxidizing bacteria were responsible for the production of N₂O in nitrification. In order to develop a unified model for better simulation of N₂O production and emissions in the biological wastewater treatment process, intensive studies should be carried out to reveal undiscovered pathways relating to N₂O production.
10

The tumor supressor function of STGC3 and its reduced expression in nasopharyngeal carcinoma

33%
He X. S., Deng M., Yang S., Xiao Z.-Q., Luo Q., He Z.-M., Hu B., Chen Z.-C.
Cellular and Molecular Biology Letters
|
2008
|
tom 13
|
nr 3
339-352
STGC3 is a novel candidate tumor suppressor gene that was found to be associated with nasopharyngeal carcinoma (NPC) via the cDNA cloning and RACE processes. The biological function of the STGC3 protein and its expression level in nasopharyngeal carcinoma remain unknown. This study aimed to evaluate the STGC3 protein expression level in NPC and to investigate the inhibitory function of STGC3 as a candidate tumor suppressor gene. We assessed the expression of the STGC3 protein in NPC biopsies and normal control specimens via Western blot and immunohistochemical analysis. The expression of STGC3 as induced by doxycycline (Dox) via a tetracycline (Tet)-regulated system in human nasopharyngeal carcinoma cell line CNE2 was also established, and the effect of STGC3 restoration on the biological behavior of CNE2 was observed. A reduced level of STGC3 expression (0.978 ± 0.213 versus 0.324 ± 0.185, P < 0.05) was detected in NPC versus normal nasopharyngeal tissue by Western blot assay. Immunohistochemical assays for STGC3 detected positive staining in the nuclei and cytoplasm of epithelial cells, and the positive expression rate in NPC, 8 of 21 (38%), was lower than that in normal nasopharynx samples, 16 of 22 (72%). After STGC3 expression was restored, the growth capacity and clone formation potential of CNE2 cells in soft agar were significantly suppressed, and the cell percentage in G0/G1 phase increased, while the percentage of cells entering the S and G2 phases decreased. This indicates that an abnormality in STGC3 expression is associated with nasopharyngeal carcinogenesis and that it may play an important role in controlling cell growth and regulating the cell cycle.
11
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Effects of organic cultivation pattern on tomato production: plant growth characteristics, quality, disease resistance, and soil physical and chemical properties

33%
Feng X., Xu Y., Liu D., Peng L., Dong J., Yao S., Feng Y., Feng Z., Li F., Hu B.
Acta Scientiarum Polonorum. Hortorum Cultus
|
2020
|
tom 19
|
nr 1
Tomato (Solanum lycopersicum L.) is one of the world’s most important cultivated vegetable. In the traditional cultivation methods, the excessive use of pesticides and fertilizers leads to an imbalance of nutrient elements in the soil, an increase in pests and diseases and a decrease in vegetable quality and yield. In the face of increasingly serious environmental and food problems, organic agriculture is considered to be an effective solution. In this experiment, the effects of organic cultivation patterns on the growth, quality, disease resistance in tomatoes, and the physical and chemical properties of soil were studied by different treatments. The results showed that the application of effective microorganisms (EM) bio-organic fertilizer in the cultivation process can significantly improve the yield, quality, and antioxidant enzyme activity of tomato. The use of straw mulching was found to significantly increase the growth, chlorophyll content, transpiration rate, and soluble sugar content of tomatoes. The application of EM bio-organic fertilizer or straw mulching significantly increased the activity of antioxidant enzyme and the expression of LeCHI gene in tomato leaves and enhanced tomato resistance to diseases. Organic production practices were found to significantly improve the soil.
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