The puropose of the study was to examine the influence of aerobic fitness on blood antioxidant system, lipid peroxidation and plasma antioxidant index response to submaximal exercise. In well-trained rowers characterized by higher aerobic fitness 30 min submaximal cycling did not affect lipid peroxidation, total oxidant status and blood antioxidant system (GSH concentration, SOD, CAT and GPX activity). However, in less aerobically trained students despite any sings of oxidative stress (lack of changes in lipid peroxidation, antioxidant index and GSH concentration) submaximal cycling induced significant decrease in blood antioxidant enzymes activities. In addition, in rowers pre-exercise blood GSH concentration and GPX activity was higher than in students. In contrast, pre-exercise blood CAT activity in rowers was lower than in students. Neither in students nor in rowers pre-exercise blood GPX, CAT and GSH concentration was related to indices of aerobic fitness. Our study revealed that 30 min submaximal cycling did not cause oxidative stress in subjects with significantly different indices of aerobic capacity.