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Activation of the intrinsic and extrinsic pathways in high pressure-induced apoptosis of murine erythroleukemia cells

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Yamaguchi T., Hashiguchi K., Katsuki S., Iwamoto W., Tsuruhara S., Terada S.

Cellular and Molecular Biology Letters

2008

tom 13

nr 1

49-57

We previously demonstrated that caspase-3, an executioner of apoptosis, is activated in the pressure-induced apoptosis of murine erythroleukemia (MEL) cells (at 100 MPa). Here, we examined the pathway of caspase-3 activation using peptide substrates and caspase inhibitors. Using the substrates of caspases-8 and -9, it was found that both are activated in cells under high pressure. The production of nuclei with sub-G1 DNA content in 100 MPa-treated MEL cells was suppressed by inhibitors of caspases-8 and -9, and pan-caspase. In 100 MPa-treated cells, pan-caspase inhibitor partially prevented the cytochrome c release from the mitochondria and the breakdown of mitochondrial membrane potential. These results suggest that the intrinsic and extrinsic pathways are activated in apoptotic signaling during the high pressure-induced death of MEL cells.

Ograniczanie wyników

1 Cellular and Molecular Biology Letters

1 Hashiguchi K.

1 Iwamoto W.

1 Katsuki S.

1 Terada S.

1 Tsuruhara S.

1 Yamaguchi T.

1 2008

Wyniki wyszukiwania

Activation of the intrinsic and extrinsic pathways in high pressure-induced apoptosis of murine erythroleukemia cells