The aim of the study was to determine the occurrence of fungi species on grain of hull-less barley grain (STH 1192, STH 1246, STH 1292, STH 4933, STH 7007 and STH 7089 – cultivar Gawrosz). The tested grain was obtained from the Plant Breeding Strzelce Ltd., IHAR group. The results were analyzed basing on the principal component analysis (PCA). It was found that the grain health condition was good. The differences in the frequency of occurrence of particular fungus species were stated between non-disinfected and disinfected grain. Over a half of the variability of the results was caused by the presence of so classified „other” fungi, and Alternaria alternata. On the other hand, 1/5 of the result variability was conditioned by the presence of species Epicoccum nigrum and Fusarium poae. The lines, which were highly infected by E. nigrum were less infected by F. poae. After surface disinfection less than 40% of the result variability was related to the occurrence of A. alternata and E. nigrum as well as the „other” fungi. Also 30% of the result variability was caused by the presence of Fusarium graminearum and F. poae, and A. alternata.