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Stress response of plant H+-PPase-expressing transgenic Escherichia coli and Saccharomyces cerevisiae: a potentially useful mechanism for the development of stress-tolerant organisms

100%

Yoon H.-S., Kim S.-Y., Kim I.-S.

Journal of Applied Genetics

2013

tom 54

nr 1

The simple proton-translocating inorganic pyrophosphatase (H+-PPase) found in plants and protists is an evolutionally conserved, essential enzyme that catalyzes the hydrolysis of pyrophosphate (PPi). Little is known about the functional contribution of H+-PPase to the cellular response to abiotic stresses, except its high salinity and drought stress. To investigate the role of H+-PPase during response to cellular stress, we isolated the cDNA of Arabidopsis thaliana H+-PPase (AVP1) and Oryza sativa H+-PPase (OVP1) and constructed transgenic Saccharomyces cerevisiae and Escherichia coli lines that express AVP1 and OVP1. In S. cerevisiae, the expression of a chimeric derivative of the AVP1 and OVP1 alleviated the phenotype associated with ipp2-deficient cells in the presence of high salinity (NaCl) and metal stressors (Cd, Mn, and Zn). In E. coli, AVP1 and OVP1 overexpression conferred enhanced tolerance to abiotic stresses, including heat shock and H2O2, as well as NaCl, Cd, Mn, Zn, Ca, and Al. Interestingly, AVP1 and OVP1 overexpression resulted in hypersensitivity to menadione and cobalt. These results demonstrate the cellular capacity of AVP1- and OVP1-expressing transgenic yeast and E. coli in response to physiological, abiotic stresses. Moreover, our results suggest new ways of engineering stress-tolerant plants that are capable of responding to climate change. Here, we provide an outline of an experimental system to examine the alternative roles of plant H+- PPase.

Oral toxicity to high level sodium fluoride causes impairment of autophagy

64%

Oka S., Li X., Zhang F., Taguchi C., Tewari N., Kim I.-S., Zhong L., Arikawa K., Liu Y., Bhawal U. K.

Journal of Physiology and Pharmacology

2020

tom 71

nr 5

Effects of LEP, GYS1, MYOD1, and MYF5 polymorphisms on pig economic traits

64%

Park S.-J., Ha J., Kim I.-S., Kwon S. G., Hwang J. H., Park D. H., Kang D. G., Kim T. W., Kim S. W., Kim C. W.

Annals of Animal Science

2015

tom 15

nr 3

Ograniczanie wyników

1 Annals of Animal Science

1 Journal of Applied Genetics

1 Journal of Physiology and Pharmacology

3 Kim I.-S.

1 Arikawa K.

1 Bhawal U. K.

1 Ha J.

1 Hwang J. H.

1 Kang D. G.

1 Kim C. W.

1 Kim S. W.

1 Kim S.-Y.

1 Kim T. W.

1 Kwon S. G.

1 Li X.

1 Liu Y.

1 Oka S.

1 Park D. H.

1 Park S.-J.

1 Taguchi C.

1 Tewari N.

1 Yoon H.-S.

1 Zhang F.

1 Zhong L.

1 2020

1 2015

1 2013

Wyniki wyszukiwania

Stress response of plant H+-PPase-expressing transgenic Escherichia coli and Saccharomyces cerevisiae: a potentially useful mechanism for the development of stress-tolerant organisms

Oral toxicity to high level sodium fluoride causes impairment of autophagy

Effects of LEP, GYS1, MYOD1, and MYF5 polymorphisms on pig economic traits