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The term adjuvant is derived from the Latin word adjuvare, which means to help. In veterinary medicine the most popular adjuvants are aluminium hydroxide and oil adjuvants, but these chemical adjuvants may cause many adverse reactions. Saponins from various herbs are mentioned among potential adjuvants. To date, saponins with adjuvant activity have been identified, among others, in Panax ginseng Astragalus species, Cochinchina momordica, Quillaja saponaria, Polygala senega, Chenopodium quinoa, and Acacia concinna. The mechanisms of immune-stimulating action of saponins have not been clearly understood. It is probable that saponins enhance cross-presentation of exogenous antigens into MHC class I pathway, thereby promoting the cellular response. Moreover, they are likely to increase the permeability of the mucosal epithelium and facilitate the entry of antigens into the bloodstream or the interaction between the mucosal-associated lymphoid tissue and antigens. Saponins induce a mixed Th₁/Th₂ response, which stimulates the production of typical cytokines (INF-γ, IL-4 or IL-10). Data presented in this article indicate clearly that saponins possess strong adjuvant properties and do not cause adverse reactions. However, a great deal of research is still needed to explain the mechanism of fitoadjuvant activity.
Problems in the reproduction of pigs may be the result of interaction of various factors, both infectious and non-infectious. Among the infectious agents, the greatest economic losses are caused by viral infection of pregnant gilts and sows. In the present study the most important pathogens causing reproductive disorders in pigs, including parvovirus (PPV), porcine reproductive and respiratory syndrome (PRRSV), swine influenza virus (SIV), porcine circovirus type 2 (PCV2), enteroviruses, encephalitis virus (EMCV), Aujeszky’s disease virus (ADV), classical swine fever virus (CSFV), Leptospira spp., Brucella suis and Erysipelotrix rhusiopathiae are characterized. So far, three possible ways of natural infection of the embryo or fetus are identified: via placenta, through the cervical canal, and by infection of the egg cell. The consequences of infection of pregnant females depend on the species of the virus, the gestation period, wherein there is an infection and immune status of pregnant females. The most common changes included: embryo death, resorption of embryos, mummification of fetuses, malformations, abortions, birth of dead or very weak piglets. Because of the importance of the reproduction sector for the competitive production of pigs, the monitoring of the health status of breeding stock, including compliance with all biosecurity rules and vaccination schedules, should be strictly respected by veterinarians taking care of pig breeding herds.
Age-related changes in serum concentrations of C-reactive protein (CRP), haptoglobin (Hp), serum amyloid A (SAA), and pig major acute phase protein (pig-MAP) were investigated in healthy pigs from birth to slaughter under field conditions. Repeated blood samples were obtained from 60 pigs at ages of 1-19 weeks. Concentrations of acute phase proteins (APP) were measured with the use of commercial ELISA kits. Concentrations of all APP increased with age (P<0.05) and positive correlations were evidenced between their concentrations and the age of pigs. Great variations in CRP, Hp, and SAA concentrations were found, as can been seen from standard deviation values. The minimal individual variability was found in regard to pig-MAP. A significant increase in all APP was observed in pigs' serum after weaning, constituting an important characteristic of this period. The elevation of APP after weaning may be associated with stress induced by mixing animals after weaning or changes in the pattern of feed administration. The peak in APP may be also caused by the initiation of synthesis of these proteins by piglets. Because a significant association between age and APP concentrations exists, further studies are needed to decide whether the age may influence the diagnostic value of APP as a marker of infection. Additionally, studies are needed to estimate whether the APP response in infection is age-dependent to any clinical importance degree.
The aim of the study was to estimate the current epidemiological situation concerning swine influenza (SI) in Poland. The study was based on an annual passive survey of 11,770 fatteners' sera from 584 herds, taken at slaughterhouses within the last 30 months (from January 2010 till June 2012), as well as, an active monitoring conducted in 2011 and 2012, in 25 farms, using 388 sera taken from life pigs of different age/technological groups. The analysis of simultaneous circulation of different swine influenza virus (SIV) subtypes was taken into a deep consideration. The wide spread of SIV in Poland, including the occurrence of multiple SIV infections was demonstrated. In 2010 and 2011, the domination of H1N1 subtype and the most frequently co-circulation of H1N1 and H1N2 viruses was evidenced, while in the first 6 months of 2012, the co-circulation of H1N1 and H3N2 viruses was detected more often. Based on the obtained results, it can be stated that the epidemiological situation concerning SI in Poland is dynamic and similar to that observed in other European regions with high pigs' density; however, the prevalence of antibodies and the occurrence of mixed SIV infections is lower than in Western Europe.
The aim of the study was to determine the effects of supplementation of sows' and weaners' diet with Stresomix, preparation containing extracts from Magnifera indica, Withania somnifera, Phyllanthus emblica, and Ocimum sanctum, on pig performance and immunity under field condition. The hypothesis was that anti-inflammatory, antistress, and immunomodulatory properties of the herbs would enhance production parameters and immune response, according to the manufacturer's claim. The study was performed on 16 sows and 160 piglets. The following parameters were recorded: concentration and proportion of white blood cells and their populations, concentration of serum immunoglobulins, specific humoral postvaccinal response after vaccination against swine influenza and swine erysipelas, and main production parameters. No significant differences among treatment groups were found with regard to concentrations of leukocyte subpopulations and immunoglobulins, as well as all investigated production parameters (P>0.05). In conclusion, the results of the study did not confirm that the investigated polyherbal product, administered at dose recommended by manufacturer, is able to significantly improve the performance and postvaccinal humoral response in clinically healthy pigs under field condition.
Pregnant gilts (in the first month of pregnancy) were infected intranasally with SIV H1N2. Clinical signs, presence of SIV RNA in nasal swabs, haemagglutination titer, hematological parameters, concentrations of interleukin (IL-10, IFNγ) and acute phase proteins (APP) (CRP, Hp), as well as production parameters, were evaluated. The concentrations of APP and cytokines were analyzed with the use of ELISA assays. Antibodies against SIV were detected with the haemagglutination inhibition assay, and the presence of RNA with RT-PCR. No clinical sings typical of swine influenza were found in the infected gilts. The gilts did not show any signs of pregnancy pathology, and no abortions were observed. No significant differences were found between the infected and control gilts with regard to the number of piglets born, hematological parameters, or the concentrations of APP and cytokines. The presence of specific antibodies against SIV was confirmed in the infected gilts starting from 14 dpi. The presence of SIV RNA in nasal swabs collected from the gilts was confirmed within 4 dpi. No SIV RNA were found in samples taken from newborn piglets.
Wild boars (Sus scrofa) are widely distributed in many countries. In recent years, their population has been expanding in most regions, despite hunting. Wild boars are known reservoirs for a number of infectious diseases that are transmissible to pigs and humans. Swine influenza (SI) is a viral respiratory disease of pigs of substantial importance both for the swine production sector and for public health. The aim of the paper is to describe the exposure to SIV of 2801 wild boars shot in 12 voivodeships of Poland from 2011 to 2014. It was assessed by the HI test. In 2011/2012, antibodies to H1N1 were found in 6.43% of wild boars’ sera. In 3.07% of sera the seroconversion to H3N2 was noted. In 2012/2013, 6.23% of sera examined were positive for H1N1, 2.67% of sera were positive for H3N2 and 3.76% of sera had antibodies to A(H1N1)2009pdm-like. In the following hunting season, antibodies to H1N1, H3N2 and A(H1N1)2009pdm-like were detected in 7.74%, 2.27% and 1.74% of samples, respectively. In 2011-2014, there were no sera samples positive for the H1N2 virus. In total, the percentage of wild boars with antibodies to SIV ranged from 0 to 6.25%, depending of the subtype. In some animals, mixed infections with different subtypes of SIV were found. In general, antibody titers were low. In summary, we confirm the exposure of wild boars’ population in Poland to SIV. On the basis of the results obtained, we suggest that their role in SIV transmission to pigs and humans has been limited. This situation may change, and therefore the monitoring of wild boars’ health status should be continued.
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