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Effects of indole-3-acetic acid and auxin transport inhibitors on the style curvature of three Alpinia species (Zingiberaceae)

100%

Luo Y.-L., Bi T.-J., Li D., Su Z.-L., Tao C., Luo Y.-J., Li Q.-J.

Acta Physiologiae Plantarum

2012

tom 34

nr 5

The effects of indole-3-acetic acid (IAA) and the auxin transport inhibitors 2, 3, 5-triiodobenzoic acid (TIBA) and 1-N-naphthylphthalamic acid (NPA) on the style curvature of Alpinia platychilus, A. blepharocalyx, and A. mutica were studied. Exogenous IAA stimulated the style curvature movement of the anaflexistylous morph (ana-morph) and cataflexistylous morph (cata-morph) of three Alpinia species in light, but had no effect in the dark. Treatment with auxin efflux inhibitors (NPA and TIBA) before flower opening did not affect the first curvature of the two morphs in darkness; however, the subsequent second movement of the ana-morph was enhanced by NPA or TIBA, while the second movement of the cata-morph was completely inhibited. After the first curvature, NPA and TIBA treatments at 06:00 hours (before significant illumination) and 11:00 hours (after the styles were illuminated for 4 h) increased the second curvature of the ana-morph, but significantly decreased that of the catamorph. The effect at 06:00 hours was more significant than the effect at 11:00 hours. These results suggested that auxin and auxin transport affected the style curvature in a different way in the two morphs, and two morphs had distinct mechanisms for style movement at different times.

Molecular characterization, expression profiles, and association analysis with hematologic parameters of the porcine HPSE and HPSE2 genes

84%

Tao C., Wang W., Zhou P., Xia T., Zhou X., Zeng C., Zhang Q., Liu B.

Journal of Applied Genetics

2013

tom 54

nr 1

Heparan sulfate (HS), which consists of repeating disaccharide units, plays an essential role in inflammation and viral infections. Heparanase (encoded by the HPSE gene) can cleave the HS chains of heparan sulfate proteoglycans (HSPGs), which are known to be important participants in immune responses. HPSE2 (heparanase 2) is a homologous gene of HPSE. To investigate the functions of HS, which is the primary receptor of the porcine reproductive and respiratory syndrome virus (PRRSV), the two genes involved in the metabolic process of HS were studied. Here, we present a study of tissue expression profiles, polymorphisms of the HPSE and HPSE2 genes, and the changes of their mRNA levels in porcine alveolar macrophages (PAMs) induced by PRRSV. Both genes are preferentially expressed in porcine immune or immune-related organs under normal conditions, e.g., in the lung, spleen, and lymph node. Moreover, a synonymous mutation c.750A>G located in exon5 of the HPSE gene was detected, and was significantly associated with the white blood cell (WBC) count, red blood cell (RBC) count, hemoglobin (HGB), and hematocrit (HCT) in the peripheral blood (p<0.05). A single nucleotide polymorphism (SNP) c.2073A>G was found in the HPSE2 gene and association analysis showed that it was significantly associated with the WBC content in the blood (p<0.05). Upon stimulation in healthy piglets with PRRSV, the HPSE mRNA was obviously up-regulated, while the HPSE2 mRNA did not induce a prominent change in PAMs.

Ograniczanie wyników

1 Acta Physiologiae Plantarum

1 Journal of Applied Genetics

2 Tao C.

1 Bi T.-J.

1 Li D.

1 Li Q.-J.

1 Liu B.

1 Luo Y.-J.

1 Luo Y.-L.

1 Su Z.-L.

1 Wang W.

1 Xia T.

1 Zeng C.

1 Zhang Q.

1 Zhou P.

1 Zhou X.

1 2013

1 2012

Wyniki wyszukiwania

Effects of indole-3-acetic acid and auxin transport inhibitors on the style curvature of three Alpinia species (Zingiberaceae)

Molecular characterization, expression profiles, and association analysis with hematologic parameters of the porcine HPSE and HPSE2 genes