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Volatile profiles and aflatoxin productionby toxigenic and non-toxigenic isolates of Aspergillus flavus grown on sterile and non-sterile cracked corn Anthony J.

100%

De Lucca A. J., Boue S. M., Carter-Wientjes C., Bhatnagar D.

Annals of Agricultural and Environmental Medicine

2012

tom 19

nr 1

Aspergillus flavus is a saprophytic fungus which can grow on corn and produce aflatoxins which render it unsafe for consumption as food and feed. In this study, aflatoxin and non-aflatoxin producing isolates of A. flavus were grown separately on wet (20% water added), sterile or non-sterile cracked corn. Wet and dry cracked corn controls were included as needed. Secondary metabolic volatiles were identified and aflatoxin concentrations determined over a 12-day period. Volatiles unique to the toxigenic A. flavus isolates were determined by comparison with volatiles produced by the respective corn controls and the non-toxigenic A. flavus isolate. The number and identity of the volatiles produced by these A. flavus isolates varied by isolate, whether sterile or non-sterile corn was the substrate, and the sampling day. Overall, most of the volatiles were produced before day 8 after inoculation. Aflatoxin production was 10-fold lower on the sterile corn, compared to the nonsterile corn. Volatiles unique to the aflatoxin producing isolates were identified on both substrates after comparison with those produced by the non-aflatoxin producing isolate, as well as the corn control samples. Results indicate that several factors (substrate, fungal isolate, culture age) affect volatile and aflatoxin production by A. flavus.

Volatile profiles of toxigenic and non-toxigenic Aspergillus flavus using SPME for solid phase extraction

76%

De L. A. J., Boue S. M., Carter-Wientjes C. H., Bland J. M., Bhatnagar D., Cleveland T. E.

Annals of Agricultural and Environmental Medicine

2010

tom 17

nr 2

301-308

Ograniczanie wyników

2 Annals of Agricultural and Environmental Medicine

2 Bhatnagar D.

2 Boue S. M.

1 Bland J. M.

1 Carter-Wientjes C.

1 Carter-Wientjes C. H.

1 Cleveland T. E.

1 De L. A. J.

1 De Lucca A. J.

1 2012

1 2010

Wyniki wyszukiwania

Volatile profiles and aflatoxin productionby toxigenic and non-toxigenic isolates of Aspergillus flavus grown on sterile and non-sterile cracked corn Anthony J.

Volatile profiles of toxigenic and non-toxigenic Aspergillus flavus using SPME for solid phase extraction