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The chloroplast peripheral reticulum (PR) is a structure of unknown function. Some authors postulated that it is a characteristic feature of C₄ plants, although it was reported from C₃ species as well. It is unknown whether the occurrence of PR follows a phylogenetic (it is found in clades containing C₄ species, regardless of the photosynthetic type) or functional (photosynthetic pathway dependent) pattern. Here, we present a phylogenetically controlled analysis of the occurrence, form and functional aspects of PR in grasses. The occurrence of the PR follows a functional and not a phylogenetic pattern. Its most elaborated form (PR type I) is a unique feature of C₄ species. Although PR was found in some of the studied C₃ grasses, it was always less developed than PR in the chloroplasts of Kranz mesophyll cells of C₄ species. The size of PR in C₄ plants was found to increase when the plants were grown under low light intensity. Additional observations, such as a negative correlation between PR size and chloroplast surface and PR occurrence in vicinity of mitochondria or plasmodesmata, suggest that PR may play some role in C₄ metabolism.
Background. Osteoporosis is a growing problem facing modern society and currently poses one of the most serious health challenges. It is a progressive skeletal disease characterised by low bone mineral density whose development depends on multiple factors. These principally include increasing age, nutrition, physical activity, endocrine changes, lifestyles, general health condition and taken drugs. Objectives. To assess how much subjects (aged >50 years) are aware of the dangers in contracting osteoporosis along with the effects that lifestyle and dairy product consumption can have on this condition developing. Material and methods. A questionnaire, designed in-house, was used to survey a group of 150 randomly selected individuals aged above 50 years. This was performed in June 2012. Questions were on socio-demographics, milk and dairy product consumption, physical activity as well as assessing knowledge about osteoporosis that included issues such as its incidence, prevention and morbidity. Results. Osteoporosis was established in 60.7% subjects of whom 69.2% were women. Only 9.3% never consumed any dairy products. Physical activity of some kind was undertaken by 77.3% subjects. Within the last year, 38.0% reported having fractures of whom 46.0% had osteoporosis and 25% were healthy. Respondents were aware that consuming dairy products is beneficial to bone health and this awareness was higher among those with osteoporosis. Physical activity was also recognised to be important in preventing this condition. Conclusions. Subjects suffering from osteoporosis had twice as many more fractures than healthy subjects.
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