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The seeds of chickpea Cicer arietinum (L.) were soaked in 0.00, 10.0 or 20.0 μg/mL of salicylic acid (SA), benzothiadiazole (BTH) or nicotinic acid (NA) solutions for 1 h. Treated seeds were sown in field and metabolite profiling of free reduced/oxidized glutathione (GSH/GSSG), SA, acetyl salicylic acid (ASA), jasmonic acid (JA), and chlorogenic acid (CGA) in chickpea crop was carried out at regular intervals (15, 30, 60, 90 and 120 days of sowing) using liquid chromatography (LC). These defense activators increased the levels of GSH, SA, and CGA in leaf; GSSG, ASA, and JA in root; and decreased the levels of GSSG and JA in leaf; GSH, SA, and CGA in root. In leaf, the biosynthesis of SA increased concomitantly with that of GSH. ASA and JA levels declined with increase of SA concentration. CGA biosynthesis increased with decline of JA level. In root, CGA and SA levels declined with increase of JA concentration. The SA level was directly related to CGA and inversely to ASA and JA concentrations. The CGA and SA concentrations increased with treatment dosage up to 120 days. The concentration of leaf GSSG was linked with JA level in root. Chickpea was a Sarich crop. GSH in leaf was the key metabolite for SA and JA signaling. The reduction in root infection and reproduction of root-knot nematode (RKN), Meloidogyne incognita was highest in BTH followed by SA and NA treatments. This was attributed to increased biosynthesis of CGA. The study firstly linked GSH and SA metabolism enhancing CGA biosynthesis in chickpea. The increase in CGA improved resistance against RKN. Seed treatment at 10.0 μg/mL BTH 1 h before sowing is recommended as strategic management decision to contain RKN on chickpea.
Seeds of four raya cvs., viz. RL 1359, RLM 198, RLM 619 and RLM 514 were desiccated for 2, 7,15 and 45 days wchich led to different levels of seed moisture. Data on germination capacity speed of germination, seedling dry weight, electrical conductance, dehydrogenase activity and seed storage behaviour for up to two years after desiccation were recorded. Based on this data, cvs. RL 1359 and RLM 514 were found to be tolerant to desiccation to as low as 0.73 and 1.16 per cent seed moisture levels, respectively. Seeds of RLM 198 tolerated desiccation up to 2.28 per cent seed moisture. However, RLM 619 seeds showed desiccation injuries in seedling dry weight, electrical conductance and speed of germination at moisture levels below 5.67 per cent.
Seed quality at physiological and harvest maturity of two pigeon pea cultivars viz. AL15 and T21 was compared. Seeds of both cultivars attained maximum dry weights, germination potential and seed vigor in terms of root/shoot lengths and dry weights of seedlings at 35 DAA in cv AL15 and 46 DAA in cv T21 denoting their physiological maturities. The levels of endogenous seed reserves like starch, proteins, lipids, DINA and RNA were comparable at physiological and harvest maturities. The seeds at physiological maturities i.e. at 35 DAA in cv AL15 and 46 DAA in cv T21 attained most of the required characters associated with the harvest of crop.
Seeds of muskmelon (Cucumis melo) cultivars viz. Pb. Hybrid and Pb. Sunehri were osmoconditioned with Polyethylene glycol j(PEG) and KNO, solutions to enhance their performance at low temperature. Osmoconditioning both with PEG 6000 and KNO3 increased per cent germination, speed of germination, vigour in terms of dry weight and length of the seedlings and root/shoot ratios. KNO3 priming recorded more pronounced effect in all the above mentioned parameters. Other vigour parameters such as electrical conductance of seed leachates was decreased with osmoconditioning while dehydrogenase activity was enhanced. Osmoconditioning resulted in increased activity of amylases.
An experiment was conducted to find out the effect of short-term heat stress on morpho-physiological characters and antioxidants in 10 diverse wheat genotypes. Seed were aseptically grown in test tubes containing filter paper whose lower half was dipped in one-fourth MS media. Heat stress conditions were created by exposing the seedlings at 45 ºC for 2 h after 7 days of their germination. Measurements were taken after 3 days of treatment. Heat stress significantly reduced the shoot dry mass, root dry mass, shoot length and root length in all the genotypes. The chlorophyll content and membrane stability index decreased, whereas proline content increased in heat-treated plants. There was significant increase in the activity of catalase, guaiacol peroxidase and superoxide dismutase under stress conditions. The genotypic variations were also significant. On the basis of a coordinated simulation of all these parameters, wheat genotypes Raj 4037 and PBW 373 were identified as tolerant to high temperature stress. The study provides evidence that the tolerant genotypes were equipped with better management of physiological processes along with an efficient antioxidative defence system, sensitivity of which can be evaluated to a sufficient level of certainty at seedling stage.
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